| Title: | GC-MS analysis of ethanol and other volatile compounds in micro-volume blood samples--quantifying neonatal exposure |
| Author(s): | Cordell RL; Pandya H; Hubbard M; Turner MA; Monks PS; |
| Address: | "Department of Chemistry, University of Leicester, Leicester, UK" |
| DOI: | 10.1007/s00216-013-6809-1 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "A static headspace gas chromatography coupled mass spectrometry (GC-MS) method was developed and fully validated for the quantitative measurement of acetaldehyde, acetone, methanol, ethanol and acetic acid in the headspace of micro-volumes of blood using n-propanol as an internal standard. The linearity of the method was established over the range 0.2-100 mg/L (R(2) > 0.99) and the limits of detection were 0.1-0.2 mg/L and lower limits quantification 0.5-1 mg/L. Precision and accuracies fell within acceptable limits (20 % for LLOQ and 15 %) for both intra- and inter-day analyses for all compounds except acetaldehyde which had inter-day variability of |
| Keywords: | "1-Propanol/blood Acetaldehyde/blood Acetates/blood Acetone/blood Ethanol/*blood Gas Chromatography-Mass Spectrometry/*methods Humans Infant, Newborn Limit of Detection Methanol/blood Sample Size Volatile Organic Compounds/*blood;" |
| Notes: | "MedlineCordell, Rebecca L Pandya, Hitesh Hubbard, Marie Turner, Mark A Monks, Paul S eng G1100158/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't Validation Study Germany 2013/02/20 Anal Bioanal Chem. 2013 May; 405(12):4139-47. doi: 10.1007/s00216-013-6809-1. Epub 2013 Feb 19" |
