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J Appl Microbiol

Title:		Tween 80 and respiratory growth affect metabolite production and membrane fatty acids in Lactobacillus casei N87
Author(s):		Zotta T; Tabanelli G; Montanari C; Ianniello RG; Parente E; Gardini F; Ricciardi A;
Address:		"Istituto di Scienze dell'Alimentazione-CNR, Avellino, Italy. Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Universita degli Studi di Bologna, Sede di Cesena, Italy. Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Universita degli Studi della Basilicata, Potenza, Italy. Dipartimento di Scienze, Universita degli Studi della Basilicata, Potenza, Italy. Dipartimento di Scienze e Tecnologie Agroalimentari, Alma Mater Studiorum, Universita degli Studi di Bologna, Cesena, Italy"
Journal Title:		J Appl Microbiol
Year:		2017
Volume:		122
Issue:		3
Page Number:		759 - 769
DOI:		10.1111/jam.13373
ISSN/ISBN:		1365-2672 (Electronic) 1364-5072 (Linking)
Abstract:		"AIMS: To evaluate the effect of cultivation (anaerobiosis vs respiration) and Tween 80 supplementation on the production of metabolites and on the composition of membrane fatty acids (FAs) in Lactobacillus casei N87. METHODS AND RESULTS: Anaerobic and respiratory growth, with or without Tween 80 supplementation, was carried out in a chemically defined medium. Production of biomass, organic acids, volatile organic compounds (VOCs), consumption of amino acids and changes in membrane FAs were investigated. Respiration altered the central metabolism rerouting pyruvate away from lactate accumulation, while Tween 80 had a minor effect on metabolic pathways. VOCs were mainly affected by growth conditions and significant amounts of diacetyl were produced by respiratory cultures. Respiration increased desaturation of membrane lipids and Tween 80 improved the production of essential polyunsaturated FAs. Palmitic acid decreased in Tween-supplemented aerated cultures. CONCLUSIONS: Combination of Tween 80 and respiratory growth promoted production of biomass and aroma compounds and affected the composition of membrane FAs in Lact. casei N87. SIGNIFICANCE AND IMPACT OF THE STUDY: Respiration might be exploited in Lact. casei as a natural strategy for the enhanced production of aroma compounds"
Keywords:		Aerobiosis Amino Acids/metabolism Anaerobiosis Fatty Acids/*metabolism Lactic Acid/metabolism Lacticaseibacillus casei/growth & development/*metabolism Metabolic Networks and Pathways/drug effects Polysorbates/*pharmacology Pyruvic Acid/metabolism Lactoba;
Notes:		"MedlineZotta, T Tabanelli, G Montanari, C Ianniello, R G Parente, E Gardini, F Ricciardi, A eng Comparative Study England 2016/12/17 J Appl Microbiol. 2017 Mar; 122(3):759-769. doi: 10.1111/jam.13373"