| Title: | Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals |
| Author(s): | Zhou YL; Zhu XQ; Gu SH; Cui HH; Guo YY; Zhou JJ; Zhang YJ; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Department of Biological Chemistry, Rothamsted Research, Harpenden AL5 2JQ, UK. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn" |
| DOI: | 10.1016/j.jinsphys.2013.10.006 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) is an agronomically important pest that causes severe economic damage to the cotton, fruit, and vegetable industries. Similar to other insects, A. lucorum can perceive and discriminate olfactory cues. A highly conserved and broadly expressed olfactory coreceptor (Orco) is crucial for insect olfaction, and Orco orthologs have been identified in several insect species. In this study, a homology-based polymerase chain reaction (PCR) method was utilized to identify AlucOrco, an Orco ortholog essential for olfaction in A. lucorum. AlucOrco shares significant sequence homology with known Orco proteins in other insects. Quantitative real-time PCR (qRT-PCR) analysis revealed that AlucOrco was abundantly expressed in adult A. lucorum. AlucOrco expression level was the highest in the antennae; by contrast, AlucOrco showed negligible expression level in other tissues. We injected AlucOrco siRNA into the conjunctivum between the prothorax and mesothorax of A. lucorum and evaluated its expression 36 h after RNA interference. The results of qRT-PCR demonstrated that the level of mRNA expression was significantly reduced (>90%) in AlucOrco siRNA-treated A. lucorum than in water-injected and non-injected controls. The electroantennogram responses of A. lucorum to two putative semiochemicals, trans-2-hexenal and trans-2-hexenyl butyrate, were also reduced significantly ( approximately 80%) in RNAi-treated A. lucorum than in the controls. These results suggest that AlucOrco is crucial in mediating odorant perception of A. lucorum, especially in perceiving trans-2-hexenal and trans-2-hexenyl butyrate" |
| Keywords: | Amino Acid Sequence Animals Arthropod Antennae/*physiology Base Sequence Electrophysiological Phenomena Female Hemiptera/*genetics Insect Proteins/metabolism Male Molecular Sequence Data Nymph/metabolism Ovum/metabolism Pheromones/*genetics Phylogeny RNA; |
| Notes: | "MedlineZhou, Yan-Le Zhu, Xiao-Qiang Gu, Shao-Hua Cui, Huan-huan Guo, Yu-Yuan Zhou, Jing-Jiang Zhang, Yong-Jun eng BB/J020281/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2013/11/13 J Insect Physiol. 2014 Jan; 60:31-9. doi: 10.1016/j.jinsphys.2013.10.006. Epub 2013 Nov 9" |
