« Previous AbstractA visual and organic vapor sensitive photonic crystal sensor consisting of polymer-infiltrated SiO2 inverse opal    Next AbstractProduction and Measurement of Organic Particulate Matter in a Flow Tube Reactor »

Mamm Genome

Title:		RNAi as a tool to control the sex ratio of mouse offspring by interrupting Zfx/Zfy genes in the testis
Author(s):		Zhang Y; Xi J; Jia B; Wang X; Wang X; Li C; Li Y; Zeng X; Ying R; Li X; Jiang S; Yuan F;
Address:		"College of Animal Science and Technology, Shihezi University, Xinjiang, Xinjiang Uygur Autonomous Region, China. Xinjiang Agricultural Vocational Technical College, Shihezi, China. College of Animal Science and Technology, Shihezi University, Xinjiang, Xinjiang Uygur Autonomous Region, China. jiabin@shzu.edu.cn. College of Life Sciences, Shihezi University, Xinjiang, Xinjiang Uygur Autonomous Region, China"
Journal Title:		Mamm Genome
Year:		2017
Volume:		20170301
Issue:		3-Apr
Page Number:		100 - 105
DOI:		10.1007/s00335-017-9682-y
ISSN/ISBN:		1432-1777 (Electronic) 0938-8990 (Linking)
Abstract:		"The objective of this study was to explore a novel method to alter the sex-ratio balance of mouse offspring by silencing the paralogous genes Zfx/Zfy (Zinc finger X/Y-chromosomal transcription factor gene) during spermatogenesis. Four recombined vectors PRZ1, PRZ2, PRZ3, and PRZ4 (RNAi-Ready-pSIREN-RetroQ-ZsGreen) were constructed for interrupting the Zfx gene. Additionally, a recombined vector Psilencer/Zfy-shRNA was constructed for interrupting the Zfy gene. Male mice were randomly divided into 8 groups, with 20 animals per group. Five groups of mice were injected with PRZ1, PRZ2, PRZ3, PRZ4, and Psilencer/Zfy-shRNA vectors, respectively. The three control groups were injected with an equal volume of physiological saline, empty RNAi-Ready-pSIREN-RetroQ-ZsGreen vector, and empty Psilencer/Zfy-shRNA vector, respectively. All groups were injected every 7 days for a total of four injections. Fourteen days after the fourth injection, 10 male mice from each group were mated individually with 10 females. Testicular tissue of 10 male mice in each group was collected, and the expression level of Zfx/Zfy mRNA was determined by qRT-PCR. Results showed that, compared with the empty RNAi-Ready-pSIREN-RetroQ-ZsGreen vector and the physiological saline group, expression of Zfx mRNA decreased significantly after injection of PRZ1 (p < 0.01), PRZ3 (p < 0.01), and PRZ4 (p < 0.01), and 78.75 +/- 7.50% of the offspring were male in PRZ4 group, significantly higher than the offspring derived from the empty RNAi-Ready-pSIREN-RetroQ-ZsGreen vector and physiological saline group (p < 0.01). In the PRZ1 group, the expression of Zfx mRNA was also significantly lower (p < 0.01), but the male rate of offspring was not different (p > 0.05). Conversely, the expression of Zfy mRNA decreased significantly after injection of Psilencer/Zfy-shRNA (p < 0.01) and 31.00 +/- 11.00% of the offspring were male, significantly lower than in the physiological saline group (p < 0.01). In conclusion, our findings show that RNAi-mediated disruption of Zfx/Zfy in mouse testis affected X/Y spermatogenesis. Additionally, results suggest that the paralogous genes Zfx/Zfy play an important role in the process of X and Y sperm development. The individual interference of Zfx/Zfy may predict the outcome of X and Y haploid sperms. Presented herein is an advanced method developed to control mouse X/Y spermatogenesis and sex ratio of offspring"
Keywords:		Animals DNA-Binding Proteins/*genetics Female Gene Silencing Kruppel-Like Transcription Factors/*genetics Male Mice RNA Interference *Sex Ratio Spermatogenesis/*genetics Spermatozoa/growth & development/metabolism Testis/growth & development/metabolism Tr;
Notes:		"MedlineZhang, YongSheng Xi, JiFeng Jia, Bin Wang, XiangZu Wang, XuHai Li, ChaoCheng Li, YaQiang Zeng, XianCun Ying, RuiWen Li, Xin Jiang, Song Yuan, FangYuan eng Research Support, Non-U.S. Gov't 2017/03/03 Mamm Genome. 2017 Apr; 28(3-4):100-105. doi: 10.1007/s00335-017-9682-y. Epub 2017 Mar 1"