| Title: | "Silent, generic and plant kairomone sensitive odorant receptors from the Southern house mosquito" |
| Author(s): | Xu P; Choo YM; Pelletier J; Sujimoto FR; Hughes DT; Zhu F; Atungulu E; Cornel AJ; Luetje CW; Leal WS; |
| Address: | "Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA" |
| DOI: | 10.1016/j.jinsphys.2013.07.004 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Print) 0022-1910 (Linking) |
| Abstract: | "The Southern house mosquito Culex quinquefasciatus has the largest repertoire of odorant receptors (ORs) of all mosquitoes and dipteran species whose genomes have been sequenced to date. Previously, we have identified and de-orphanized two ORs expressed in female antennae, CquiOR2 and CquiOR10, which are sensitive to oviposition attractants. In view of a new nomenclature for the Culex genome (VectorBase) we renamed these ORs as CquiOR21 (formerly CquiOR10) and CquiOR121 (CquiOR2). In addition, we selected ORs from six different phylogenetic groups for deorphanization. We cloned four of them by using cDNA from female antennae as a template. Attempts to clone CquiOR87 and CquiOR110 were unsuccessful either because they are pseudogenes or are not expressed in adult female antennae, the main olfactory tissue. By contrast, CquiOR1, CquiOR44, CquiOR73, and CquiOR161 were highly expressed in female antennae. To de-orphanize these ORs, we employed the Xenopus oocyte recording system. CquiORx-CquiOrco-expressed oocytes were challenged with a panel of 90 compounds, including known oviposition attractants, human and vertebrate host odorants, plant kairomones, and naturally occurring repellents. While CquiOR161 did not respond to any test compound in two different laboratories, CquiOR1 showed the features of a generic OR, with strong responses to 1-octen-3-ol and other ligands. CquiOR44 and CquiOR73 showed preference to plant-derived terpenoids and phenolic compounds, respectively. While fenchone was the best ligand for the former, 3,5-dimethylphenol elicited the strongest responses in the latter. The newly de-orphanized ORs may be involved in reception of plant kairomones and/or natural repellents" |
| Keywords: | "Animals Arthropod Antennae/*metabolism Culex/*genetics/metabolism Eugenol/metabolism Female Male Pheromones/metabolism Phylogeny Receptors, Odorant/*genetics/metabolism Terminology as Topic Xenopus CquiOR1 CquiOR121 CquiOR161 CquiOR21 CquiOR44 CquiOR73 Cu;" |
| Notes: | "MedlineXu, Pingxi Choo, Young-Moo Pelletier, Julien Sujimoto, Fernando R Hughes, David T Zhu, Fen Atungulu, Elizabeth Cornel, Anthony J Luetje, Charles W Leal, Walter S eng R01 AI095514/AI/NIAID NIH HHS/ R01 DC011091/DC/NIDCD NIH HHS/ R01AI095514/AI/NIAID NIH HHS/ R01DC011091/DC/NIDCD NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2013/07/24 J Insect Physiol. 2013 Sep; 59(9):961-6. doi: 10.1016/j.jinsphys.2013.07.004. Epub 2013 Jul 19" |
