| Title: | Two O-methyltransferases isolated from flower petals of Rosa chinensis var. spontanea involved in scent biosynthesis |
| Author(s): | Wu S; Watanabe N; Mita S; Ueda Y; Shibuya M; Ebizuka Y; |
| Address: | "Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan" |
| ISSN/ISBN: | 1389-1723 (Print) 1347-4421 (Linking) |
| Abstract: | "Rosa chinensis var. spontanea predominantly emits 1,3,5-trimethoxybenzene together with methyleugenol and isomethyleugenol as minor floral scent compounds. Two O-methyltransferases (OMTs), designated as RcOMT1 and RcOMT2, were isolated from rose flower petals using homology-based screening strategies. RcOMT1 efficiently methylated eugenol and isoeugenol to yield volatile methyleugenol and isomethyleugenol, respectively. Furthermore, the mRNA transcripts of RcOMT1 were highly expressed in floral organs and the expression pattern coincided with intracellular content changes of methyleugenol and isomethyleugenol in rose flowers. In contrast, RcOMT2, which shows 94% similarity with caffeic acid O-methyltransferase (COMT) of Prunus amygdalus, was expressed in all tissues tested and had the highest activity with caffeic acid, a typical substrate for COMT. However, this COMT-like OMT also showed some degrees of activity with all three putative precursors of 1,3,5-trimethoxybenzene" |
| Notes: | "PubMed-not-MEDLINEWu, Shuiqin Watanabe, Naoharu Mita, Satoru Ueda, Yoshihiro Shibuya, Masaaki Ebizuka, Yutaka eng Japan 2005/10/20 J Biosci Bioeng. 2003; 96(2):119-28" |
