| Title: | Post-translational transport of proteins into microsomal membranes of Candida maltosa |
| Author(s): | Wiedmann M; Wiedmann B; Voigt S; Wachter E; Muller HG; Rapoport TA; |
| Address: | "Zentralinstitut fur Molekularbiologie, Akademie der Wissenschaften der DDR, Berlin-Buch" |
| DOI: | 10.1002/j.1460-2075.1988.tb03006.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "We have isolated from the yeast Candida maltosa microsomal membranes that are active in the translocation of proteins synthesized in cell-free systems derived from C. maltosa, Saccharomyces cerevisiae or wheat germ. Translocation and core glycosylation of prepro-alpha-factor, a secretory protein, were observed with yeast microsomes added during or after translation. The signal peptide is cleaved off. Cytochrome P-450 from C. maltosa, the first integral membrane protein studied in a yeast system, is also inserted both co- and post-translationally into Candida microsomal membranes. Its insertion into canine microsomes occurs efficiently only in a co-translational manner and is dependent on the function of the signal recognition particle" |
| Keywords: | "Candida/*metabolism Cell-Free System Fungal Proteins/*metabolism Glycosylation Intracellular Membranes/metabolism Mating Factor Membrane Proteins/*metabolism Microsomes/*metabolism Peptides/metabolism Protein Precursors/metabolism Protein Processing, Post;" |
| Notes: | "MedlineWiedmann, M Wiedmann, B Voigt, S Wachter, E Muller, H G Rapoport, T A eng England 1988/06/01 EMBO J. 1988 Jun; 7(6):1763-8. doi: 10.1002/j.1460-2075.1988.tb03006.x" |
