| Title: | "The sex pheromone precursor androsta-5,16-dien-3 beta-ol is a major early metabolite in in vitro pregnenolone metabolism in human testicular homogenates" |
| Author(s): | Weusten JJ; Smals AG; Hofman JA; Kloppenborg PW; Benraad TJ; |
| Address: | "Department of Medicine, St. Radboud Hospital, University of Nijmegen, The Netherlands" |
| ISSN/ISBN: | 0021-972X (Print) 0021-972X (Linking) |
| Abstract: | "In an earlier report we described the early time sequence of the in vitro metabolism of [4-14C]pregnenolone ([4-14C]P5) to testosterone in homogenates of human and rat testes and demonstrated the appearance of mainly delta 5 (humans)- and delta 4 (rats)-steroids within minutes after starting the incubation. In this study strong evidence is presented for the substantial synthesis from P5 of the sex pheromone precursor androsta-5,16-dien-3 beta-ol (ADL) in human, but not rat, testicular homogenates. The 16-unsaturated C19 steroid ADL appeared after 1 min of incubation, and within 5 min reached values (17-23% of total radioactivity added as [4-14C]P5) comparable to those of the major delta 5-steroids 17 alpha-hydroxypregnenolone and dehydroepiandrosterone. Thus, in humans, as in boars, the sex attractant precursor ADL is a major early testicular metabolite of P5" |
| Keywords: | "Androstenols/isolation & purification/*metabolism Animals Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Humans In Vitro Techniques Male Pheromones/*metabolism Pregnenolone/*metabolism Rats Rats, Inbred Strains Sex Attractants/*;" |
| Notes: | "MedlineWeusten, J J Smals, A G Hofman, J A Kloppenborg, P W Benraad, T J eng 1987/10/01 J Clin Endocrinol Metab. 1987 Oct; 65(4):753-6. doi: 10.1210/jcem-65-4-753" |
