| Title: | "Over-expression of the Saccharomyces cerevisiae exo-beta-1,3-glucanase gene together with the Bacillus subtilis endo-beta-1,3-1,4-glucanase gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in yeast" |
| Author(s): | van Rensburg P; van Zyl WH; Pretorius IS; |
| Address: | "Institute for Wine Biotechnology, University of Stellenbosch, South Africa" |
| DOI: | 10.1016/s0168-1656(97)00059-x |
| ISSN/ISBN: | 0168-1656 (Print) 0168-1656 (Linking) |
| Abstract: | "The EXG1 gene encoding the main Saccharomyces cerevisiae exo-beta-1,3-glucanase was cloned and over-expressed in yeast. The Bacillus subtilis endo-1,3-1,4-beta-glucanase gene (beg1) and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene (end1) were fused to the secretion signal sequence of the yeast mating pheromone alpha-factor (MF alpha 1S) and inserted between the yeast alcohol dehydrogenase II gene promoter (ADH2P) and terminator (ADH2T). Constructs ADH2P-MF alpha 1S-beg1-ADH2T and ADH2P-MF alpha 1S-end 1-ADH2T designated BEG1 and END1, respectively, were expressed separately and jointly with EXG1 in S. cerevisiae. The construction of fur 1 ura3 S. cerevisiae strains allowed for the autoselection of these multicopy URA3-based plasmids in rich medium. Enzyme assays confirmed that co-expression of EXG1, BEG1 and END1 enhanced glucan degradation by S. cerevisiae" |
| Keywords: | "Amino Acid Sequence Bacillus subtilis/enzymology/*genetics Bacteroidaceae/enzymology/*genetics Cloning, Molecular *Gene Expression Glucan 1, 3-beta-Glucosidase Glucans/metabolism Glycoside Hydrolases/biosynthesis/*genetics/metabolism Molecular Sequence Dat;" |
| Notes: | "Medlinevan Rensburg, P van Zyl, W H Pretorius, I S eng Research Support, Non-U.S. Gov't Netherlands 1997/05/23 J Biotechnol. 1997 May 23; 55(1):43-53. doi: 10.1016/s0168-1656(97)00059-x" |
