| Title: | Sexual agglutination in Saccharomyces cerevisiae |
| DOI: | 10.1128/jb.148.3.889-896.1981 |
| ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
| Abstract: | "Treatment of either mating type of Saccharomyces cerevisiae with the appropriate sex pheromone increased cell-cell binding in a modified cocentrifugation assay. Constitutive agglutination of haploids was qualitatively similar to pheromone-induced agglutination. Regardless of exposure to pheromone, agglutinable combinations of cells exhibited maximal binding across similar ranges of ionic strength, pH, and temperature. Binding of all combinations was inhibited by 8 M urea, 1 M pyridine, or 0.05% sodium dodecyl sulfate. From alpha-cells we solubilized and partially purified an inhibitor of a-cell agglutinability. This inhibitor reversibly masked all a-cell adhesion sites and inactivated pheromone-treated and control cells with similar kinetics. The inhibitor behaved as a homogeneous species in heat inactivation experiments. Based on these results, we proposed a model for pheromone effects on agglutination in S. cerevisiae" |
| Keywords: | "Agglutination *Agglutinins Hydrogen-Ion Concentration Mating Factor Models, Biological Osmolar Concentration Peptides/*pharmacology Saccharomyces cerevisiae/analysis/*physiology Temperature;" |
| Notes: | "MedlineTerrance, K Lipke, P N eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1981/12/01 J Bacteriol. 1981 Dec; 148(3):889-96. doi: 10.1128/jb.148.3.889-896.1981" |
