| Title: | The responses of Vitreoscilla hemoglobin-expressing hybrid aspen (Populus tremula x tremuloides) exposed to 24-h herbivory: expression of hemoglobin and stress-related genes in exposed and nonorthostichous leaves |
| Author(s): | Sutela S; Ylioja T; Jokipii-Lukkari S; Anttila AK; Julkunen-Tiitto R; Niemi K; Mollari T; Kallio PT; Haggman H; |
| Address: | "Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland, suvi.sutela@oulu.fi" |
| DOI: | 10.1007/s10265-013-0569-z |
| ISSN/ISBN: | 1618-0860 (Electronic) 0918-9440 (Linking) |
| Abstract: | "The responses of transcriptome and phenolic compounds were determined with Populus tremula L. x Populus tremuloides Michx. expressing the hemoglobin (Hb) of Vitreoscilla (VHb) and non-transformant (wt) line. After 24-h exposure of leaves to Conistra vaccinii L., the transcript levels of endogenous non-symbiotic class 1 Hb (PttHb1) and truncated Hb (PttTrHb) genes were modestly reduced and increased, respectively, in both wt and VHb-expressing line. Besides the herbivory exposed leaves showing the most significant transcriptome changes, alterations were also detected in the transcriptome of nonorthostichous leaves positioned directly above the exposed leaves. Both wt and VHb-expressing line displayed similar herbivory-induced effects on gene expression, although the extent of responses was more pronounced in the wt than in the VHb-expressing line. The contents of phenolic compounds were not altered due to herbivory and they were alike in the wt and VHb-expressing line. In addition, we determined the relative growth rates (RGRs) of Orthosia gothica L., Ectropis crepuscularia Denis & Schiff. and Orgyia antiqua L. larvae, and found no variation in the RGRs between the lines. Thus, VHb-expressing P. tremula x tremuloides lines showed to be comparable with wt in regards to the food quality of leaves" |
| Keywords: | "Animals Bacterial Proteins/*genetics Chimera Gene Expression Gene Expression Profiling *Gene Expression Regulation, Plant Herbivory Hydroxybenzoates/analysis Insecta/physiology Larva Oligonucleotide Array Sequence Analysis Plant Leaves/genetics/parasitolo;" |
| Notes: | "MedlineSutela, Suvi Ylioja, Tiina Jokipii-Lukkari, Soile Anttila, Anna-Kaisa Julkunen-Tiitto, Riitta Niemi, Karoliina Mollari, Tiina Kallio, Pauli T Haggman, Hely eng Research Support, Non-U.S. Gov't Japan 2013/06/08 J Plant Res. 2013 Nov; 126(6):795-809. doi: 10.1007/s10265-013-0569-z. Epub 2013 Jun 7" |
