| Title: | "Continuously perfusable, customisable, and matrix-free vasculature on a chip platform" |
| Author(s): | Chesnais F; Joel J; Hue J; Shakib S; Di Silvio L; Grigoriadis AE; Coward T; Veschini L; |
| Address: | "Academic Centre of Reconstructive Science, Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. francois.chesnais@kcl.ac.uk. Centre for Craniofacial and Regenerative Medicine, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK" |
| ISSN/ISBN: | 1473-0189 (Electronic) 1473-0189 (Linking) |
| Abstract: | "Creating vascularised cellular environments in vitro is a current challenge in tissue engineering and a bottleneck towards developing functional stem cell-derived microtissues for regenerative medicine and basic investigations. Here we have developed a new workflow to manufacture vasculature on chip (VoC) systems efficiently, quickly, and inexpensively. We have employed 3D printing for fast-prototyping of bespoke VoC and coupled them with a refined organotypic culture system (OVAA) to grow patent capillaries in vitro using tissue-specific endothelial and stromal cells. Furthermore, we have designed and implemented a pocket-size flow driver to establish physiologic perfusive flow throughout our VoC-OVAA with minimal medium use and waste. Using our platform, we have created vascularised microtissues and perfused them at physiologic flow rates for extended time (>2 weeks) observing flow-dependent vascular remodelling. Overall, we present for the first time a scalable and customisable system to grow vascularised and perfusable microtissues, a key initial step to grow mature and functional tissues in vitro. We envision that this technology will empower fast prototyping and validation of increasingly biomimetic in vitro systems, including interconnected multi-tissue systems" |
| Keywords: | *Volatile Organic Compounds Tissue Engineering Perfusion Lab-On-A-Chip Devices; |
| Notes: | "MedlineChesnais, Francois Joel, Jordan Hue, Jonas Shakib, Sima Di Silvio, Lucy Grigoriadis, Agamemnon E Coward, Trevor Veschini, Lorenzo eng Research Support, Non-U.S. Gov't England 2023/02/02 Lab Chip. 2023 Feb 14; 23(4):761-772. doi: 10.1039/d2lc00930g" |
