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Reprod Domest Anim

Title:		Salivary luteinizing hormone: An open window to detect oestrous period in buffalo
Author(s):		Srinivasan M; Muthukumar S; Saibaba G; Manikkaraja C; Abdulkader Akbarsha M; Archunan G;
Address:		"Pheromone Technology Laboratory, Department of Animal Science, Bharathidasan University, Tiruchirappalli, India. School of Chemical and Biotechnology, SASTRA University, Thanjavur, India. Department of Animal Science, Volcani Center, Agricultural Research Organization, Rishon LeTzion, Israel. Research Co-ordinator, National College (Autonomous), Tiruchirappalli, India"
Journal Title:		Reprod Domest Anim
Year:		2020
Volume:		20200220
Issue:		5
Page Number:		647 - 651
DOI:		10.1111/rda.13649
ISSN/ISBN:		1439-0531 (Electronic) 0936-6768 (Linking)
Abstract:		"Silent oestrus is an unsurmountable problem in the management of buffalo reproduction. In addressing this issue, we have earlier reported variation in the levels of urinary luteinizing hormone (LH) through the different phases of oestrous cycle with an extended window during the mid-oestrous phase. Based on this report, the present study is designed to assess the salivary LH levels in buffalo during the different phases of oestrous cycle. Bovine LH ELISA kit was used to determine the level of salivary LH. We observed a notable variation in salivary LH levels during the different phases of oestrous cycle. The maximum LH level, 39.07 mIU/ml, observed during oestrus, which was significantly (p < .05) higher than other consecutive phases. Altogether, the results showed a significant (p < .05) fold variation during oestrus compared with other phases. Therefore, the study convincingly shows that salivary LH has the potential of application in development of a modality for non-invasive oestrous detection in buffalo"
Keywords:		Animals Buffaloes/*physiology Enzyme-Linked Immunosorbent Assay/veterinary Estrous Cycle Estrus/*physiology Female Luteinizing Hormone/*analysis Saliva/*chemistry Elisa artificial insemination non-invasive oestrous cycle oestrous detection ovulation saliv;
Notes:		"MedlineSrinivasan, Mahalingam Muthukumar, Subramanian Saibaba, Ganesan Manikkaraja, Chidambaram Abdulkader Akbarsha, Mohammad Archunan, Govindaraju eng ICAR-National Fund/ DST-FIST-Phase-I/ DST-PURSE/ Germany 2020/01/29 Reprod Domest Anim. 2020 May; 55(5):647-651. doi: 10.1111/rda.13649. Epub 2020 Feb 20"