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Arch Med Res

Title:		Yeast as an expression system for the production of bacterial and viral antigens
Author(s):		Silva A; Benitez J;
Address:		"Facultad de Biologia, Universidad de La Habana, Cuba"
Journal Title:		Arch Med Res
Year:		1994
Volume:		25
Issue:		4
Page Number:		447 - 449
DOI:
ISSN/ISBN:		0188-4409 (Print) 0188-4409 (Linking)
Abstract:		"To develop a yeast system that expresses bacterial and viral antigens in the future, we analyzed yeast secretion with a reporter system based on the cc1A gene of Clostridium thermocellum that encodes endoglucanase A. Sequences coding for the mature CelA protein were fused in frame to yeast promoters and secretion signals, introduced into different vectors and analyzed biochemically in yeast secretory mutants. Gene dosage had little effect, whereas expression from a weak promoter enhanced endoglucanase A secretion. In yeast secretory mutants, a major fraction of endoglucanase A piled up in the endoplasmic reticulum as a core glycosylated protein"
Keywords:		"Antigens, Bacterial/*genetics Antigens, Viral/*genetics Blotting, Northern Cellulase/genetics Cloning, Molecular/*methods Clostridium/enzymology/genetics Mating Factor Peptides/genetics Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineSilva, A Benitez, J eng 1994/01/01 Arch Med Res. 1994 Winter; 25(4):447-9"