| Title: | Regulated secretion of MuGM-CSF in Saccharomyces cerevisiae via GAL1:MF alpha 1 prepro sequences |
| Author(s): | Shaw KJ; Frommer BR; Anagnost JA; Narula S; Leibowitz PJ; |
| Address: | "Schering Corporation, Bloomfield, NJ 07003" |
| ISSN/ISBN: | 0198-0238 (Print) 0198-0238 (Linking) |
| Abstract: | "Murine granulocyte-macrophage colony-stimulating factor (GM-CSF) was expressed in Saccharomyces cerevisiae using a novel regulated secretion system. This system involves the fusion of the GAL1 upstream regulatory region to the signal sequence of the alpha mating pheromone, and the integration of this GAL1:MF alpha 1 prepro:MuGM-CSF construct into the yeast chromosome. These constructs were very stable under both selective and nonselective conditions: after 30 generations of growth no plasmid loss was observed. The expression and secretion of MuGM-CSF were analyzed by biological assays and Western blots of yeast culture medium and yeast cell extracts. Expression of MuGM-CSF was regulated by galactose induction. In addition, expression levels were proportional to the number of tandem copies of the gene inserted into the yeast chromosome" |
| Keywords: | "Animals Colony-Stimulating Factors/*genetics DNA/genetics Gene Expression Regulation Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances/*genetics Immunochemistry Mice Plasmids *Promoter Regions, Genetic Saccharomyces cerevi;" |
| Notes: | "MedlineShaw, K J Frommer, B R Anagnost, J A Narula, S Leibowitz, P J eng 1988/03/01 DNA. 1988 Mar; 7(2):117-26. doi: 10.1089/dna.1988.7.117" |
