| Title: | The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system |
| Author(s): | Scotter JM; Allardyce RA; Langford VS; Hill A; Murdoch DR; |
| Address: | "Syft Technologies Ltd., Medical Applications, Christchurch, New Zealand. jenny.scotter@syft.com" |
| DOI: | 10.1016/j.mimet.2005.09.016 |
| ISSN/ISBN: | 0167-7012 (Print) 0167-7012 (Linking) |
| Abstract: | "We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms" |
| Keywords: | Automation Bacteremia/*microbiology Bacteria/*growth & development Bacteriological Techniques/instrumentation/methods Blood/*microbiology *Culture Media Humans Mass Spectrometry/instrumentation/methods Time Factors; |
| Notes: | "MedlineScotter, J M Allardyce, R A Langford, V S Hill, A Murdoch, D R eng Comparative Study Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2005/11/08 J Microbiol Methods. 2006 Jun; 65(3):628-31. doi: 10.1016/j.mimet.2005.09.016. Epub 2005 Nov 3" |
