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J Agric Food Chem

Title:		New Insights into Stress-Induced beta-Ocimene Biosynthesis in Tea (Camellia sinensis) Leaves during Oolong Tea Processing
Author(s):		Chen S; Xie P; Li Y; Wang X; Liu H; Wang S; Han W; Wu R; Li X; Guan Y; Yang Z; Yu X;
Address:		"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Institute of Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521, United States"
Journal Title:		J Agric Food Chem
Year:		2021
Volume:		20210923
Issue:		39
Page Number:		11656 - 11664
DOI:		10.1021/acs.jafc.1c04378
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"As the major contributors to the floral odors of tea products, terpenoid volatiles play critical roles in the defense response of plants to multiple stresses. Until now, only a few TPS genes in tea plants (Camellia sinensis) have been functionally validated. In this study, by comparative studies conducted at gene, protein, and metabolite levels during oolong tea processing, we isolated an ocimene synthase gene, CsOCS, which displays a low similarity to previously characterized tea ocimene synthases. Further prokaryotic expression and subcellular localization analysis showed that it is plastid-located and could produce (E)-beta-ocimene and (Z)-beta-ocimene using GPP as the substrate. The optimum temperature and pH of the enzyme were 30 degrees C and 7.5, respectively. Treatment with exogenous methyl jasmonate elevated the transcript level of CsOCS and enhanced the emission of ocimene from tea leaves. Collectively, CsOCS is implicated as a key enzyme for beta-ocimene synthesis during oolong tea processing"
Keywords:		Acyclic Monoterpenes Alkenes *Camellia sinensis Plant Leaves Plant Proteins/genetics Tea jasmonates ocimene oolong tea stress response tea processing terpene synthase;
Notes:		"MedlineChen, Si Xie, Peifeng Li, Yeye Wang, Xiaxia Liu, Huihui Wang, Shanshan Han, Wenbo Wu, Ruimei Li, Xinlei Guan, Yuefeng Yang, Zhenbiao Yu, Xiaomin eng 2021/09/24 J Agric Food Chem. 2021 Oct 6; 69(39):11656-11664. doi: 10.1021/acs.jafc.1c04378. Epub 2021 Sep 23"