« Previous AbstractPlant volatile organic compounds (VOCs) in ozone (O3) polluted atmospheres: the ecological effects    Next AbstractQuality of the Indoor Environment in Elderly Care Centers in Two Cities in Central Portugal: Viseu and Covilha »

J Proteome Res

Title:		Urinary Volatilomics Unveils a Candidate Biomarker Panel for Noninvasive Detection of Clear Cell Renal Cell Carcinoma
Author(s):		Pinto J; Amaro F; Lima AR; Carvalho-Maia C; Jeronimo C; Henrique R; Bastos ML; Carvalho M; Guedes de Pinho P;
Address:		"UCIBIO/REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Cancer Biology & Epigenetics Group-Research Centre, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, Portugal. Department of Pathology, Portuguese Oncology Institute of Porto, 4200-072 Porto, Portugal. Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar-University of Porto (ICBAS-UP), 4050-313 Porto, Portugal. Fernando Pessoa Energy, Environment and Health Research Unit (FP-ENAS), Faculty of Health Sciences, University Fernando Pessoa, 4249-004 Porto, Portugal"
Journal Title:		J Proteome Res
Year:		2021
Volume:		20210402
Issue:		6
Page Number:		3068 - 3077
DOI:		10.1021/acs.jproteome.0c00936
ISSN/ISBN:		1535-3907 (Electronic) 1535-3893 (Linking)
Abstract:		"Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk of systemic progression. Hence, reliable molecular biomarkers of ccRCC are needed to provide early and minimally invasive detection. In this study, urinary volatilome profiling of patients diagnosed with ccRCC (n = 75), and cancer-free controls (n = 75), was performed to investigate the presence of a volatile signature characteristic of ccRCC. Volatile organic compounds (VOCs) in general, and more specifically volatile carbonyl compounds (VCCs), present in urine were extracted by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Supervised multivariate models showed a good discriminatory power of ccRCC patients from controls in urine. Overall, 22 volatile metabolites were found significantly altered between the two groups, including aldehydes, ketones, aromatic hydrocarbons, and terpenoids. A candidate six-biomarker panel, comprising octanal, 3-methylbutanal, benzaldehyde, 2-furaldehyde, 4-heptanone, and p-cresol, depicted the best performance for ccRCC detection with 83% sensitivity, 79% specificity, and 81% accuracy. Moreover, the ccRCC urinary volatilome signature suggested dysregulation of energy metabolism and overexpression of enzymes associated with carcinogenesis. These findings provide the molecular basis for the fine-tuning of gas-sensing materials for application in the development of a bioelectronic sensor"
Keywords:		"Biomarkers *Carcinoma, Renal Cell/diagnosis Gas Chromatography-Mass Spectrometry Humans *Kidney Neoplasms/diagnosis Solid Phase Microextraction *Volatile Organic Compounds/analysis clear cell renal cell carcinoma diagnostic biomarkers urine volatilome;"
Notes:		"MedlinePinto, Joana Amaro, Filipa Lima, Ana Rita Carvalho-Maia, Carina Jeronimo, Carmen Henrique, Rui Bastos, Maria de Lourdes Carvalho, Marcia Guedes de Pinho, Paula eng Research Support, Non-U.S. Gov't 2021/04/03 J Proteome Res. 2021 Jun 4; 20(6):3068-3077. doi: 10.1021/acs.jproteome.0c00936. Epub 2021 Apr 2"