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EMBO J

Title:		Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex
Author(s):		Panek HR; Stepp JD; Engle HM; Marks KM; Tan PK; Lemmon SK; Robinson LC;
Address:		"LSU Medical Center, Department of Biochemistry and Molecular Biology, Shreveport, LA 71130, USA"
Journal Title:		EMBO J
Year:		1997
Volume:		16
Issue:		14
Page Number:		4194 - 4204
DOI:		10.1093/emboj/16.14.4194
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"In Saccharomyces cerevisiae, the redundant YCK1 and YCK2 genes (Yeast Casein Kinase 1) are required for viability. We describe here the molecular analysis of four mutations that eliminate the requirement for Yck activity. These mutations alter proteins that resemble the four subunits of clathrin adaptors (APs), with highest sequence similarity to those of the recently identified AP-3 complex. The four yeast subunits are associated in a high-molecular-weight complex. These proteins have no essential function and are not redundant for function with other yeast AP-related proteins. Combination of suppressor mutations with a clathrin heavy chain mutation (chc1-ts) confers no synthetic growth defects. However, a yck(ts) mutation shows a strong synthetic growth defect with chc1-ts. Moreover, endocytosis of Ste3p is dramatically decreased in yck(ts) cells and is partially restored by the AP suppressor mutations. These results suggest that vesicle trafficking at the plasma membrane requires the activity of Yck protein kinases, and that the new AP-related complex may participate in this process"
Keywords:		"Adaptor Proteins, Vesicular Transport Blotting, Western *Casein Kinase I Casein Kinases Cell Division Clathrin/genetics/metabolism DNA Mutational Analysis Endocytosis Endosomes/metabolism Membrane Proteins/metabolism Molecular Sequence Data *Monomeric Cla;"
Notes:		"MedlinePanek, H R Stepp, J D Engle, H M Marks, K M Tan, P K Lemmon, S K Robinson, L C eng 39040/PHS HHS/ AG00105/AG/NIA NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. England 1997/07/16 EMBO J. 1997 Jul 16; 16(14):4194-204. doi: 10.1093/emboj/16.14.4194"