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J Bacteriol

Title:		"The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10"
Author(s):		Nakayama J; Ruhfel RE; Dunny GM; Isogai A; Suzuki A;
Address:		"Department of Agricultural Chemistry, University of Tokyo, Japan"
Journal Title:		J Bacteriol
Year:		1994
Volume:		176
Issue:		23
Page Number:		7405 - 7408
DOI:		10.1128/jb.176.23.7405-7408.1994
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"Conjugative transfer of the Enterococcus faecalis tetracycline resistance plasmid pCF10 is stimulated by a peptide pheromone, cCF10. Once a recipient strain acquires pCF10 and thus becomes a pheromone-responsive donor, cCF10 activity is no longer detected in culture filtrates. Here we show that pCF10 encodes a peptide inhibitor, iCF10, secreted by donor cells; this inhibitor antagonizes the cCF10 activity in culture filtrates. In order to detect and quantitate iCF10, we developed a reverse-phase high-performance liquid chromatography assay in which the inhibitor peptide elutes separately from the pheromone; this type of assay enabled us to determine that lack of pheromone activity in donor culture filtrates was due to secretion of a mixture of iCF10 and cCF10, rather than abolition of cCF10 secretion. The gene encoding iCF10, prgQ, is located on the EcoRI-C fragment of pCF10. The open reading frame comprising the prgQ gene encodes a 23-amino-acid precursor that resembles a signal peptide. This precursor is cleaved to the mature heptapeptide iCF10 during the secretion process"
Keywords:		"Amino Acid Sequence Bacterial Proteins/*genetics/pharmacology Base Sequence Cloning, Molecular Conjugation, Genetic/drug effects Enterococcus faecalis/*genetics Genes, Bacterial/*genetics Molecular Sequence Data Oligopeptides/*antagonists & inhibitors/pha;"
Notes:		"MedlineNakayama, J Ruhfel, R E Dunny, G M Isogai, A Suzuki, A eng GM49530/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/12/01 J Bacteriol. 1994 Dec; 176(23):7405-8. doi: 10.1128/jb.176.23.7405-7408.1994"