| Title: | Vortex-ultrasound-assisted dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry for the analysis of volatile bioactive components and comparative pharmacokinetic study of the herb-herb interactions in Guanxin Shutong Capsule |
| Author(s): | Mu J; Gao X; Li Q; Yang X; Yang W; Sun X; Bi K; Zhang H; |
| Address: | "School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, China" |
| ISSN/ISBN: | 1615-9314 (Electronic) 1615-9306 (Linking) |
| Abstract: | "Guanxin Shutong Capsule, an effective traditional Chinese medicine, is widely used for coronary heart disease clinically. Volatile components are one of its important bioactive constituents. To better understand the material basis for the therapeutic effects, the components of Guanxin Shutong Capsule absorbed into the blood and their metabolites were identified based on gas chromatography with mass spectrometry coupled with vortex-ultrasound-assisted dispersive liquid-liquid microextraction. As a result, three prototypes and 15 metabolites were identified or tentatively characterized in rat plasma. Subsequently, a pharmacokinetic study was carried out to monitor the concentrations of the main bioactive constituents and metabolites (isoborneol, borneol, eugenol, and camphor) by gas chromatography with mass spectrometry in rat plasma following oral administration of single herb extract and different combinations of herbs in this prescription. Compared to other groups, a statistically significant difference of the pharmacokinetic properties was obtained when the total complex prescription was administered, indicating possible drug-drug interactions among the complex ingredients of Guanxin Shutong Capsule. These findings provided an experimental basis concerning the clinical application and medicinal efficacy of Guanxin Shutong Capsule in the treatment of coronary heart disease" |
| Keywords: | "Animals Drugs, Chinese Herbal/*analysis/*pharmacokinetics Gas Chromatography-Mass Spectrometry Herb-Drug Interactions Liquid Phase Microextraction Plasma/chemistry Rats Volatile Organic Compounds/*analysis Traditional Chinese medicine comparative pharmaco;" |
| Notes: | "MedlineMu, Jingqing Gao, Xun Li, Qing Yang, Xiaomei Yang, Wenling Sun, Xu Bi, Kaishun Zhang, Huifen eng Germany 2017/06/20 J Sep Sci. 2017 Aug; 40(16):3267-3278. doi: 10.1002/jssc.201700500. Epub 2017 Jul 17" |
