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Mol Cell Biol

Title:		Substitutions in the hydrophobic core of the alpha-factor receptor of Saccharomyces cerevisiae permit response to Saccharomyces kluyveri alpha-factor and to antagonist
Author(s):		Marsh L;
Address:		"Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461"
Journal Title:		Mol Cell Biol
Year:		1992
Volume:		12
Issue:		9
Page Number:		3959 - 3966
DOI:		10.1128/mcb.12.9.3959-3966.1992
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in hydrophobic segments in the core of the receptor (M54I, S145L, S145L-S219L, A229V, L255S-S288P). Remarkably, strains expressing these mutant receptors exhibited positive pheromone responses to desTrp1,Ala3-alpha-factor, an analog that normally blocks these responses. The M54I mutation appeared to affect only ligand specificity. The other mutations conferred additional effects on signaling or recovery. Two mutants were more sensitive to alpha-factor than wild type (S145L, A229V). One mutant was more sensitive to alpha-factor-induced cell cycle arrest initially, but then recovered more efficiently (S145L-S219L). One mutant (L255S-S288P) conferred positive pheromone responses to alpha-factor as assayed by FUS1-lacZ reporter induction, but did not display growth arrest. The hydrophobic receptor core thus appears to control activation by some ligands and to play roles in aspects of signal transduction and recovery"
Keywords:		"Amino Acid Sequence Mating Factor Molecular Sequence Data Mutation Peptides/antagonists & inhibitors/*metabolism Receptors, Cell Surface/genetics/*metabolism Receptors, Mating Factor *Receptors, Peptide Saccharomyces/*metabolism Saccharomyces cerevisiae/g;"
Notes:		"MedlineMarsh, L eng AI18738/AI/NIAID NIH HHS/ GM43365/GM/NIGMS NIH HHS/ P30 CA13330/CA/NCI NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1992/09/01 Mol Cell Biol. 1992 Sep; 12(9):3959-66. doi: 10.1128/mcb.12.9.3959-3966.1992"