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Environ Sci Pollut Res Int

Title:		"Production, identification, and field evaluation of sex pheromone from calling females in Diaphania angustalis (Lepidoptera: Crambidae)"
Author(s):		Ma T; Liu Z; Wang C; Zhang S; Shi X; Sun Z; Chen X; Jia C; Wang C; He Y; Wen X;
Address:		"Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. , Wutongshan Park, Shenzhen, 518114, China. Department of Entomology, Rutgers University, New Brunswick, NJ, 08901, USA. College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. wenxiujun@scau.edu.cn"
Journal Title:		Environ Sci Pollut Res Int
Year:		2017
Volume:		20170912
Issue:		31
Page Number:		24485 - 24493
DOI:		10.1007/s11356-017-0119-7
ISSN/ISBN:		1614-7499 (Electronic) 0944-1344 (Linking)
Abstract:		"Insect sex pheromones play a crucial role in the mate finding and calling behavior of Lepidoptera pests. Currently, little is known about the chemical ecology of Diaphania angustalis Snellen (Lepidoptera: Crambidae), a severe and important defoliator attacking the medicinal plant, Alstonia scholaris. In the present study, the pheromone components of D. angustalis females were investigated using electrophysiological and behavioral methods. Distilled hexane extracts of female pheromone glands were analyzed through electroantennogram (EAG) and gas chromatography-electroantennogram detector (GC-EAD), and the active compounds were identified through gas chromatography-mass spectrometry (GC-MS). Production peak of female sex pheromone occurred on the third day of age at 5 h into the scotophase with the EAG test, and the hexane extracts were attractive to males in the wind tunnel test. GC-EAD analysis of virgin males to gland extracts that were subsequently evaluated showed two active compounds, (E,E)-10,12-hexadecadienal (E10E12-16:Ald) and (E,E)-10,12-hexadecadien-1-ol (E10E12-16:OH), based on comparison of retention time and mass spectrum, with suitable synthetic compounds. Under laboratory conditions, the blend of E10E12-16:Ald and E10E12-16:OH in a ratio of 9:1 elicited a stronger EAG response than other treatments or a single component. In the field, more male moths were captured by traps baited with the mixture of E10E2-16:Ald and E10E2-16:OH in a ratio of 9:1, whereas a mixture of 8:1 and 10:1 also caught males. Accordingly, E10E2-16:Ald and E10E2-16:OH were regarded as the major sex pheromone components in D. angustalis females"
Keywords:		Animals *Chemotaxis Electrophysiological Phenomena Female Gas Chromatography-Mass Spectrometry Male Moths/chemistry/*physiology Sex Attractants/chemistry/*metabolism Diaphania angustalis Field bioassay Identification Production Sex pheromone;
Notes:		"MedlineMa, Tao Liu, Zhitao Wang, Cai Zhang, Shengnan Shi, Xianhui Sun, Zhaohui Chen, Xiaoyang Jia, Caijuan Wang, Changlu He, Yurong Wen, Xiujun eng Germany 2017/09/14 Environ Sci Pollut Res Int. 2017 Nov; 24(31):24485-24493. doi: 10.1007/s11356-017-0119-7. Epub 2017 Sep 12"