| Title: | Simultaneous quantification of 11 active constituents in Shexiang Baoxin Pill by ultraperformance convergence chromatography combined with tandem mass spectrometry |
| Author(s): | Lv C; Chen L; Fu P; Yang N; Liu Q; Xu Y; Sun Q; Li R; Zhan C; Zhang W; Liu R; |
| Address: | "School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, PR China. School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Department of Nephrology, the 455th Hospital of PLA, Shanghai 200052, PR China. Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China. School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Waters Corporation, Building 13, No. 1000 JinHai Road, Pudong New District, Shanghai 201206, PR China. Department of Nephrology, the 455th Hospital of PLA, Shanghai 200052, PR China. Shanghai Hutchison Pharmaceuticals Company, Shanghai 200331, PR China. School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, PR China. Electronic address: wdzhangy@hotmail.com. School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: lyliurh@126.com" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2017.03.033 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "On account of the complexity of chemical constituents of Shexiang Baoxin Pill (SBP), a famous traditional Chinese medicine (TCM) formula, a novel and effective UPC(2)-MS/MS method was developed to simultaneously determine the content of 11 active compounds of SBP with outstanding separation ability. Eleven components in SBP, including 2 ginsenosides, 2 bile acids, 3 bufadienolides and 4 volatiles were detected by electrospray ionization tandem mass spectrometry in positive and negative ion modes with multiple reaction monitor (MRM). The analysis was performed at 30 degrees C using an Acquity UPC(2) Diol (3.0x50mm, 1.7mum) column with linear gradient elution (eluent A, CO(2); eluent B, methanol containing 20mM ammonium acetate), back pressure of 2000 psi, flow rate of 1.2mL/min and the injection volume of 1.0muL. The method was extensively validated regarding the linearity (r>/=0.9974), precision ( |
| Keywords: | "Bile Acids and Salts/analysis Bufanolides/analysis Chromatography, High Pressure Liquid/economics/*methods Drugs, Chinese Herbal/*chemistry Ginsenosides/analysis Limit of Detection Quality Control Reproducibility of Results Tandem Mass Spectrometry/econom;" |
| Notes: | "MedlineLv, Chao Chen, Liqing Fu, Peng Yang, Niao Liu, Qun Xu, Yongwei Sun, Qinglong Li, Rui Zhan, Changsen Zhang, Weidong Liu, Runhui eng Validation Study Netherlands 2017/04/07 J Chromatogr B Analyt Technol Biomed Life Sci. 2017 May 1; 1052:135-141. doi: 10.1016/j.jchromb.2017.03.033. Epub 2017 Mar 31" |
