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Beitr Tab Int

Title:		Gas Chromatography-Tandem Mass Spectrometry Method for Selective Detection of 2-Nitropropane in Mainstream Cigarette Smoke
Author(s):		Chapman GM; Junco JG; Cardenas RB; Watson CH; Valentin-Blasini L;
Address:		"Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341, USA. Oak Ridge Institute for Science and Education, 1299 Bethel Valley Rd, Oak Ridge, TN 37830, USA"
Journal Title:		Beitr Tab Int
Year:		2019
Volume:		28
Issue:		7
Page Number:		300 - 309
DOI:		10.2478/cttr-2019-0011
ISSN/ISBN:		0173-783X (Print) 1612-9237 (Electronic) 0173-783X (Linking)
Abstract:		"Although 2-nitropropane is a potentially harmful compound present in cigarette smoke, there are few fully-validated, modern methods to quantitate it in mainstream cigarette smoke. We developed an isotope dilution gas chromatography-tandem mass spectrometry (ID-GC-MS/MS) method for the detection of 2-nitropropane in mainstream cigarette smoke. The vapor fraction of mainstream cigarette smoke was collected in inert polyvinyl fluoride gas sampling bags and extracted with hexanes containing isotopically labeled internal standard, then purified and concentrated via solid-phase extraction using a normal phase silica adsorbent and a 100% dichloromethane eluant. This method is sensitive enough to measure vapor phase 2-nitropropane concentrations in the nanogram range, with a 19 ng per cigarette method limit of detection. Product variability estimated from the analysis of 15 cigarette products yielded relative standard deviations ranging from 5.4% to 15.7%, and estimates of precision from two quality control products yielded relative standard deviations of 9.49% and 14.9%. Under the Health Canada Intense smoking regimen, 2-nitropropane in machine-generated mainstream smoke from 15 cigarette products ranged from 98.3 to 363 ng per cigarette"
Keywords:		2-Nitropropane gas chromatography mass spectrometry tobacco volatile organic compound;
Notes:		"PubMed-not-MEDLINEChapman, Gala M Junco, Juliana Giraldo Cardenas, Roberto Bravo Watson, Clifford H Valentin-Blasini, Liza eng CC999999/ImCDC/Intramural CDC HHS/ Poland 2019/12/01 Beitr Tab Int. 2019 Dec 1; 28(7):300-309. doi: 10.2478/cttr-2019-0011"