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J Neurosci

Title:		Transduction for pheromones in the main olfactory epithelium is mediated by the Ca2+ -activated channel TRPM5
Author(s):		Lopez F; Delgado R; Lopez R; Bacigalupo J; Restrepo D;
Address:		"Department of Biology, Faculty of Sciences, University of Chile, Santiago 1058, Chile, Department of Cell and Developmental Biology, Neuroscience Program, and Rocky Mountain Taste and Smell Center, Anschutz Medical Campus, University of Colorado, Aurora, Colorado 80045"
Journal Title:		J Neurosci
Year:		2014
Volume:		34
Issue:		9
Page Number:		3268 - 3278
DOI:		10.1523/JNEUROSCI.4903-13.2014
ISSN/ISBN:		1529-2401 (Electronic) 0270-6474 (Print) 0270-6474 (Linking)
Abstract:		"Growing evidence suggests that the main olfactory epithelium contains a subset of olfactory sensory neurons (OSNs) responding to pheromones. One candidate subpopulation expresses the calcium activated cation channel TRPM5 (transient receptor potential channel M5). Using GFP driven by the TRPM5 promoter in mice, we show that this subpopulation responds to putative pheromones, urine, and major histocompatibility complex peptides, but not to regular odors or a pheromone detected by other species. In addition, this subpopulation of TRPM5-GFP+ OSNs uses novel transduction. In regular OSNs, odorants elicit activation of the cyclic nucleotide-gated (CNG) channel, leading to Ca2+ gating of Cl- channels; in TRPM5-GFP+ OSNs, the Ca2+ -activated Cl- ANO2 (anoctamin 2) channel is not expressed, and pheromones elicit activation of the CNG channel leading to Ca2+ gating of TRPM5. In conclusion, we show that OSNs expressing TRPM5 respond to pheromones, but not to regular odors through the opening of CNG channels leading to Ca2+ gating of TRPM5"
Keywords:		Animals Anoctamins Calcium/*metabolism Chloride Channels/metabolism Enzyme Inhibitors/pharmacology Gene Expression Regulation/drug effects/genetics Green Fluorescent Proteins Histocompatibility Antigens/chemistry Ion Channel Gating/drug effects/genetics M;Neuroscience;
Notes:		"MedlineLopez, Fabian Delgado, Ricardo Lopez, Roberto Bacigalupo, Juan Restrepo, Diego eng P30 DC004657/DC/NIDCD NIH HHS/ R01 DC006070/DC/NIDCD NIH HHS/ R03 TW007920/TW/FIC NIH HHS/ Research Support, Non-U.S. Gov't 2014/02/28 J Neurosci. 2014 Feb 26; 34(9):3268-78. doi: 10.1523/JNEUROSCI.4903-13.2014"