| Title: | Screening candidate genes related to volatile synthesis in shiitake mushrooms and construction of regulatory networks to effectively improve mushroom aroma |
| Author(s): | Li W; Chen WC; Wang JB; Feng J; Wu D; Zhang Z; Zhang JS; Yang Y; |
| Address: | "Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, People's Republic of China, Shanghai Guosen Bio-tech Co. Ltd, 1000 Jinqi Road, Shanghai, China. Institute of Biotechnology Research, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, China" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: Metabolite formation is a physiological stress response during the growth and development of shiitake mushrooms (Lentinula edodes). The characteristic flavor metabolites are important quality components in shiitake mushrooms. To investigate the formation mechanisms of characteristic flavor metabolites, transcriptome analyses were performed on shiitake mushrooms harvested at different growth stages. RESULTS: In total, 30 genes related to the synthesis of characteristic volatiles of mushrooms were identified via screening. Through KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of the selected genes and correlation analyses of gene expressions, the main volatile synthesis pathways were determined as histidine metabolism, glutathione metabolism and biosynthesis of unsaturated fatty acids. Gene cluster and correlation analyses were performed to clarify the combined effects of different genes in the enzymatic reactions. Further, a correlation network of candidate genes was built based on the gene expression levels. CONCLUSION: The activities of flavor synthases and the content of characteristic flavor metabolites were analyzed; the enzyme activity changes and metabolic product distribution sites were clarified. A synthesis and regulation network was constructed for the candidate genes and characteristic volatiles, and information was obtained for 16 hub genes. Moreover, it was essential to identify and characterize the key genes and synthases involved in the synthesis of the characteristic volatiles of mushrooms. This information provides us with a better understanding of the biosynthesis and regulation of the volatiles, which will lay the foundation for improving the quality of shiitake mushrooms. (c) 2021 Society of Chemical Industry" |
| Keywords: | Biosynthetic Pathways Flavoring Agents/chemistry/*metabolism Fungal Proteins/*genetics/metabolism Gene Regulatory Networks Odorants/analysis Shiitake Mushrooms/chemistry/*genetics/metabolism Volatile Organic Compounds/chemistry/metabolism expression level; |
| Notes: | "MedlineLi, Wen Chen, Wan-Chao Wang, Jin-Bin Feng, Jie Wu, Di Zhang, Zhong Zhang, Jing-Song Yang, Yan eng 31901812/National Natural Science Foundation of China/ England 2021/03/13 J Sci Food Agric. 2021 Oct; 101(13):5618-5626. doi: 10.1002/jsfa.11213. Epub 2021 Mar 27" |
