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Appl Biochem Biotechnol


Title:Additional paper waste in pulping sludge for biohydrogen production by heat-shocked sludge
Author(s):Chairattanamanokorn P; Tapananont S; Detjaroen S; Sangkhatim J; Anurakpongsatorn P; Sirirote P;
Address:"Environmental Technology Unit, Department Environmental Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. fscippck@ku.ac.th"
Journal Title:Appl Biochem Biotechnol
Year:2012
Volume:20111119
Issue:2
Page Number:389 - 401
DOI: 10.1007/s12010-011-9434-5
ISSN/ISBN:1559-0291 (Electronic) 0273-2289 (Linking)
Abstract:"Dark anaerobic fermentation is an interesting alternative method for producing biohydrogen (H(2)) as a renewable fuel because of its low cost and various usable organic substrates. Pulping sludge from wastewater treatment containing plentiful cellulosic substrate could be feasibly utilized for H(2) production by dark fermentation. The objective of this study was to investigate the optimal proportion of pulping sludge to paper waste, the optimal initial pH, and the optimal ratio of carbon and nitrogen (C/N) for H(2) production by anaerobic seed sludge pretreated with heat. The pulping sludge was pretreated with NaOH solution at high temperature and further hydrolyzed with crude cellulase. Pretreatment of the pulping sludge with 3% NaOH solution under autoclave at 121 degrees C for 2 h, hydrolysis with 5 FPU crude cellulase at 50 degrees C, and pH 4.8 for 24 h provided the highest reducing sugar production yield (229.68 +/- 2.09 mg/g(TVS)). An initial pH of 6 and a C/N ratio of 40 were optimal conditions for H(2) production. Moreover, the supplement of paper waste in the pulping sludge enhanced the cumulative H(2) production yield. The continuous hydrogen production was further conducted in a glass reactor with nylon pieces as supporting media and the maximum hydrogen production yield was 151.70 ml/g(TVS)"
Keywords:*Bioreactors Carbon/metabolism Fermentation *Hot Temperature Hydrogen/*metabolism Hydrogen-Ion Concentration Hydrolysis *Industrial Waste Nitrogen/metabolism *Paper *Sewage Volatile Organic Compounds/metabolism;
Notes:"MedlineChairattanamanokorn, Prapaipid Tapananont, Supachok Detjaroen, Siriporn Sangkhatim, Juthatip Anurakpongsatorn, Patana Sirirote, Pramote eng Research Support, Non-U.S. Gov't 2011/11/22 Appl Biochem Biotechnol. 2012 Jan; 166(2):389-401. doi: 10.1007/s12010-011-9434-5. Epub 2011 Nov 19"

 
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