| Title: | Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC((R)) in mite allergic subjects with asthma |
| Author(s): | Khayath N; Doyen V; Gherasim A; Radu C; Choual I; Beck N; Jacob A; Schoettel F; Vecellio L; Domis N; de Blay F; |
| Address: | "Chest Diseases Department, Strasbourg University Hospital, Strasbourg, France. Federation of Translational Medicine EA 3070, University of Strasbourg, Strasbourg, France. Clinics of Immuno-Allergology, CHU Brugmann, Universite Libre de Bruxelles (ULB), Brussels, Belgium. Biocluster des Haras, ALYATEC(R), Strasbourg, France. Francois-Rabelais University, Tours Cedex, France" |
| DOI: | 10.1080/02770903.2018.1563902 |
| ISSN/ISBN: | 1532-4303 (Electronic) 0277-0903 (Linking) |
| Abstract: | "Objective: Environmental Exposure Chamber (EEC) should have standardized and controlled allergenic and non-allergenic exposures to perform reproducible clinical studies. The aim was to demonstrate that mite exposure in the Alyatec((R)) EEC could induce early (EAR) and/or late asthmatic reactions (LAR) in at least 60% of subjects allergic to mite.Methods: The EEC has a volume of 147-m(3) with 20 seats. The nebulized particle number, airborne Der p1, endotoxins, and volatile organic compound (VOC) concentrations were measured. Twenty-four asthmatics allergic to mite were randomly exposed to 15, 25, and 46 ng/m(3) Der p1. Specificity was assessed in not mite-sensitized asthmatics.Results: No significant endotoxin or VOC contamination was measured. The mean inter-assay CVs were 12.5% for the airborne particle number and 28.7% for airborne Der p1 concentrations. For the three Der p1 concentrations, at least 88% of the subjects developed EAR and/or LAR, and at least 46% developed a dual response. No reaction occurred with placebo or in the control group. No severe bronchial reaction occurred.Conclusions: The Alyatec((R)) EEC demonstrated a tight control of allergenic and non-allergenic exposures. The EEC was clinically validated, with airborne Der p1 levels close to levels found in natural settings" |
| Keywords: | "Adolescent Adult Animals Antigens, Dermatophagoides/pharmacology Arthropod Proteins/pharmacology Asthma/*epidemiology Cross-Over Studies Cysteine Endopeptidases/pharmacology Double-Blind Method Endotoxins/pharmacology Environmental Exposure Female Humans;" |
| Notes: | "MedlineKhayath, Naji Doyen, Virginie Gherasim, Alina Radu, Carmen Choual, Ibrahim Beck, Nicole Jacob, Audrey Schoettel, Florian Vecellio, Laurent Domis, Nathalie de Blay, Frederic eng Randomized Controlled Trial Research Support, Non-U.S. Gov't England 2019/03/29 J Asthma. 2020 Feb; 57(2):140-148. doi: 10.1080/02770903.2018.1563902. Epub 2019 Mar 28" |
