| Title: | The regulation of Delta11-desaturase gene expression in the pheromone gland of Mamestra brassicae (Lepidoptera; Noctuidae) during pheromonogenesis |
| Author(s): | Koblos G; Danko T; Sipos K; Geiger A; Szlanka T; Fodor J; Fonagy A; |
| Address: | "Department of Pathophysiology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman Otto ut 15, H-1022 Budapest, Hungary. Ecotoxicology and Environmental Analysis Group, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman Otto ut 15, H-1022 Budapest, Hungary. Department of Entomology, Faculty of Horticultural Science, Corvinus University of Budapest, H-1118 Menesi ut, 44, H-1118 Budapest, Hungary. Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Hungary. Ecotoxicology and Environmental Analysis Group, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman Otto ut 15, H-1022 Budapest, Hungary. Electronic address: fonagy.adrien@agrar.mta.hu" |
| DOI: | 10.1016/j.ygcen.2015.03.004 |
| ISSN/ISBN: | 1095-6840 (Electronic) 0016-6480 (Linking) |
| Abstract: | "Cabbage moth (Mamestra brassicae) females produce sex pheromones to attract conspecific males. In our M. brassicae colony, the pheromone blend is composed of Z11-hexadecenyl acetate (Z11-16Ac) and hexadecyl acetate (16Ac) in a 93:7 ratio. A fatty acyl Delta11-desaturase is involved in the production of the main pheromone component. The release of Pheromone Biosynthesis Activating Neuropeptide (PBAN) regulates the pheromone production in the pheromone gland (PG). We cloned a cDNA encoding the MambrDelta11-desaturase and analyzed its expression profile over time in M. brassicae tissues. Transcript levels of the Delta11-desaturase in larvae, pupal PGs, fat body, brain and muscle tissues were <0.1% of that in female PGs, whereas expression in male genitalia was 2%. In the PGs of virgin females the expression level increased continuously from eclosion to the end of the 1st day when it reached a plateau without further significant fluctuation up to the 8th day. In contrast, we recorded a characteristic daily rhythmicity in pheromone production with a maximum around 200 ng Z11-16Ac/PG. In some experiments, females were decapitated to prevent PBAN release and thereby inhibit pheromone production, which remarkably increased after treatment with Mambr-Pheromonotropin. Further experiments revealed that mating resulted in a significant suppression of pheromone production. However, expression of the Delta11-desaturase was not affected by any of these interventions, suggesting that it's not regulated by PBAN. Fluorescent microscopy was used to study the potential role of lipid droplets during pheromone production, however, no lipid droplets were identified indicating that pheromonogenesis is regulated via de novo fatty acid synthesis" |
| Keywords: | "Animals Cloning, Molecular Fatty Acid Desaturases/genetics/*metabolism Female Gene Expression Regulation/*drug effects Insect Proteins/genetics/*metabolism Larva/cytology/drug effects/metabolism Lipids/analysis Male Moths/*enzymology/genetics/growth & dev;" |
| Notes: | "MedlineKoblos, Gabriella Danko, Tamas Sipos, Kitti Geiger, Agnes Szlanka, Tamas Fodor, Jozsef Fonagy, Adrien eng Research Support, Non-U.S. Gov't 2015/03/23 Gen Comp Endocrinol. 2015 Sep 15; 221:217-27. doi: 10.1016/j.ygcen.2015.03.004. Epub 2015 Mar 18" |
