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Biosens Bioelectron

Title:		Peptide receptor-based selective dinitrotoluene detection using a microcantilever sensor
Author(s):		Hwang KS; Lee MH; Lee J; Yeo WS; Lee JH; Kim KM; Kang JY; Kim TS;
Address:		"Center for Biomicrosystem, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea. kshwang@kist.re.kr"
Journal Title:		Biosens Bioelectron
Year:		2011
Volume:		20110922
Issue:		1
Page Number:		249 - 254
DOI:		10.1016/j.bios.2011.09.021
ISSN/ISBN:		1873-4235 (Electronic) 0956-5663 (Linking)
Abstract:		"We reported that peptide could be utilized as receptor molecule in the gas phase for application in micro/nano sensors by using a specific peptide that recognizes 2,4-dinitrotoluene at room temperature and in an atmospheric environment and measuring changes in the resonant frequency of the peptide immobilized microcantilevers. By using these peptides as receptors on a microcantilever sensor, we were able to experimentally detect 2,4-dinitrotoluene (DNT) vapor at concentrations as low as parts per billion (ppb) in the gas phase. While resonant frequency changes after binding between 2,4-DNT and the specific peptide receptor that was immobilized on microcantilevers were observed, the resonant frequency of DNT nonspecific peptide immobilized microcantilever did not change when exposed to 2,4-DNT vapor. The limit of detection (LOD) was calculated to be 431 ppt of limit of detection is numerically expected by experimental based on an equation that describes the relationship between the noise-equivalent analyte concentration. These results indicate that the peptide receptors hold great promise for use in the development of an artificial olfactory system and electronic nose based on micro/nanotechnology for monitoring various chemical vapors in the gas phase such as explosive mixtures of chemicals and/or volatile organic compounds"
Keywords:		"Biosensing Techniques/*instrumentation Equipment Design Equipment Failure Analysis Explosive Agents/*analysis Micro-Electrical-Mechanical Systems/*instrumentation Microchemistry/*instrumentation Receptors, Peptide/*chemistry Trinitrotoluene/*analysis;"
Notes:		"MedlineHwang, Kyo Seon Lee, Min Hyuck Lee, Juhee Yeo, Woon-Seok Lee, Jeong Hoon Kim, Kang-Min Kang, Ji Yoon Kim, Tae Song eng Research Support, Non-U.S. Gov't England 2011/10/18 Biosens Bioelectron. 2011 Dec 15; 30(1):249-54. doi: 10.1016/j.bios.2011.09.021. Epub 2011 Sep 22"