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Metab Eng

Title:		Production of moth sex pheromones for pest control by yeast fermentation
Author(s):		Holkenbrink C; Ding BJ; Wang HL; Dam MI; Petkevicius K; Kildegaard KR; Wenning L; Sinkwitz C; Lorantfy B; Koutsoumpeli E; Franca L; Pires M; Bernardi C; Urrutia W; Mafra-Neto A; Ferreira BS; Raptopoulos D; Konstantopoulou M; Lofstedt C; Borodina I;
Address:		"The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, 2800 Kgs. Lyngby, Denmark; BioPhero ApS, Lerso Parkalle 42-44, 2100, Copenhagen, Denmark. Lund University, Department of Biology, Solvegatan 37, SE-223 62, Lund, Sweden. The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, 2800 Kgs. Lyngby, Denmark; Lund University, Department of Biology, Solvegatan 37, SE-223 62, Lund, Sweden. BioPhero ApS, Lerso Parkalle 42-44, 2100, Copenhagen, Denmark. Chemical Ecology and Natural Products Laboratory, Institute of Biosciences and Applications, National Centre of Scientific Research Demokritos, Attikis, Greece. Biotrend S.A., Biocant Park, Nucleo 04 Lote 2, 3060-197, Cantanhede, Portugal. ISCA Technologies, 1230 W. Spring St. Riverside, California, 92507, USA. Novagrica Hellas S.A, TESPA 'Lefkippos', 15341, Athens, Greece. Lund University, Department of Biology, Solvegatan 37, SE-223 62, Lund, Sweden. Electronic address: christer.lofstedt@biol.lu.se. The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, 2800 Kgs. Lyngby, Denmark; BioPhero ApS, Lerso Parkalle 42-44, 2100, Copenhagen, Denmark. Electronic address: irbo@biosustain.dtu.dk"
Journal Title:		Metab Eng
Year:		2020
Volume:		20201009
Issue:
Page Number:		312 - 321
DOI:		10.1016/j.ymben.2020.10.001
ISSN/ISBN:		1096-7184 (Electronic) 1096-7176 (Linking)
Abstract:		"The use of insect sex pheromones is an alternative technology for pest control in agriculture and forestry, which, in contrast to insecticides, does not have adverse effects on human health or environment and is efficient also against insecticide-resistant insect populations. Due to the high cost of chemically synthesized pheromones, mating disruption applications are currently primarily targeting higher value crops, such as fruits. Here we demonstrate a biotechnological method for the production of (Z)-hexadec-11-en-1-ol and (Z)-tetradec-9-en-1-ol, using engineered yeast cell factories. These unsaturated fatty alcohols are pheromone components or the immediate precursors of pheromone components of several economically important moth pests. Biosynthetic pathways towards several pheromones or their precursors were reconstructed in the oleaginous yeast Yarrowia lipolytica, which was further metabolically engineered for improved pheromone biosynthesis by decreasing fatty alcohol degradation and downregulating storage lipid accumulation. The sex pheromone of the cotton bollworm Helicoverpa armigera was produced by oxidation of fermented fatty alcohols into corresponding aldehydes. The resulting yeast-derived pheromone was just as efficient and specific for trapping of H. armigera male moths in cotton fields in Greece as a conventionally produced synthetic pheromone mixture. We further demonstrated the production of (Z)-tetradec-9-en-1-yl acetate, the main pheromone component of the fall armyworm Spodoptera frugiperda. Taken together our work describes a biotech platform for the production of commercially relevant titres of moth pheromones for pest control via yeast fermentation"
Keywords:		Animals Fermentation Humans Male *Moths Pest Control *Sex Attractants *Yarrowia Fatty acyl-CoA desaturase Fatty alcohols Lepidoptera Yarrowia lipolytica;
Notes:		"MedlineHolkenbrink, Carina Ding, Bao-Jian Wang, Hong-Lei Dam, Marie Inger Petkevicius, Karolis Kildegaard, Kanchana Rueksomtawin Wenning, Leonie Sinkwitz, Christina Lorantfy, Bettina Koutsoumpeli, Eleni Franca, Lucas Pires, Marina Bernardi, Carmem Urrutia, William Mafra-Neto, Agenor Ferreira, Bruno Sommer Raptopoulos, Dimitris Konstantopoulou, Maria Lofstedt, Christer Borodina, Irina eng Research Support, Non-U.S. Gov't Belgium 2020/10/13 Metab Eng. 2020 Nov; 62:312-321. doi: 10.1016/j.ymben.2020.10.001. Epub 2020 Oct 9"