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J Dairy Sci

Title:		Short communication: Practical issues in implementing volatile metabolite analysis for identifying mastitis pathogens
Author(s):		Hettinga KA; de Bok FA; Lam TJ;
Address:		"Dairy Science and Technology, Food Quality and Design group, Wageningen University, Bornse Weilanden 9, 6708 WG, Wageningen, the Netherlands. Electronic address: kasper.hettinga@wur.nl. KBBL Wijhe BV, Industrieweg 16, 8131 VZ Wijhe, the Netherlands. GD Animal Health, 7400 AA Deventer, the Netherlands; Department of Farm Animal Health, Utrecht University, PO Box 80151, 3508 TD Utrecht, the Netherlands"
Journal Title:		J Dairy Sci
Year:		2015
Volume:		20150903
Issue:		11
Page Number:		7906 - 7910
DOI:		10.3168/jds.2015-9720
ISSN/ISBN:		1525-3198 (Electronic) 0022-0302 (Linking)
Abstract:		"Several parameters for improving volatile metabolite analysis using headspace gas chromatography-mass spectrometry (GC-MS) analysis of volatile metabolites were evaluated in the framework of identification of mastitis-causing pathogens. Previous research showed that the results of such volatile metabolites analysis were comparable with those based on bacteriological culturing. The aim of this study was to evaluate the effect of several method changes on the applicability and potential implementation of this method in practice. The use of a relatively polar column is advantageous, resulting in a faster and less complex chromatographic setup with a higher resolving power yielding higher-quality data. Before volatile metabolite analysis is applied, a minimum incubation of 8h is advised, as reducing incubation time leads to less reliable pathogen identification. Application of GC-MS remained favorable compared with regular gas chromatography. The complexity and cost of a GC-MS system are such that this limits the application of the method in practice for identification of mastitis-causing pathogens"
Keywords:		"Animals Cattle Chromatography, Gas Escherichia coli/*isolation & purification/metabolism Female Flame Ionization/veterinary Gas Chromatography-Mass Spectrometry/*veterinary Mastitis, Bovine/*diagnosis/microbiology Milk/*chemistry Staphylococcus/*isolation;"
Notes:		"MedlineHettinga, Kasper A de Bok, Frank A M Lam, Theo J G M eng Evaluation Study Research Support, Non-U.S. Gov't 2015/09/08 J Dairy Sci. 2015 Nov; 98(11):7906-10. doi: 10.3168/jds.2015-9720. Epub 2015 Sep 3"