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Anal Chem

Title:		Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope
Author(s):		Harada T; Yuba-Kubo A; Sugiura Y; Zaima N; Hayasaka T; Goto-Inoue N; Wakui M; Suematsu M; Takeshita K; Ogawa K; Yoshida Y; Setou M;
Address:		"Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto 619-0237, Japan"
Journal Title:		Anal Chem
Year:		2009
Volume:		81
Issue:		21
Page Number:		9153 - 9157
DOI:		10.1021/ac901872n
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"We have developed a mass microscope (mass spectrometry imager with spatial resolution higher than the naked eye) equipped with an atmospheric pressure ion-source chamber for laser desorption/ionization (AP-LDI) and a quadrupole ion trap time-of-flight (QIT-TOF) analyzer. The optical microscope combined with the mass spectrometer permitted us to precisely determine the relevant tissue region prior to performing imaging mass spectrometry (IMS). An ultraviolet laser tightly focused with a triplet lens was used to achieve high spatial resolution. An atmospheric pressure ion-source chamber enables us to analyze fresh samples with minimal loss of intrinsic water or volatile compounds. Mass-microscopic AP-LDI imaging of freshly cut ginger rhizome sections revealed that 6-gingerol ([M + K](+)at m/z 333.15, positive mode; [M - H](-) at m/z 293.17, negative mode) and the monoterpene ([M + K](+) at m/z 191.09), which are the compounds related to pungency and flavor, respectively, were localized in oil drop-containing organelles. AP-LDI-tandem MS/MS analyses were applied to compare authentic signals from freshly cut ginger directly with the standard reagent. Thus, our atmosphere-imaging mass spectrometer enabled us to monitor a quality of plants at the organelle level"
Keywords:		Atmospheric Pressure Catechols/analysis Fatty Alcohols/analysis Ginger/chemistry Monoterpenes/analysis Tandem Mass Spectrometry/*instrumentation/methods Volatile Organic Compounds/*analysis/chemistry;
Notes:		"MedlineHarada, Takahiro Yuba-Kubo, Akiko Sugiura, Yuki Zaima, Nobuhiro Hayasaka, Takahiro Goto-Inoue, Naoko Wakui, Masatoshi Suematsu, Makoto Takeshita, Kengo Ogawa, Kiyoshi Yoshida, Yoshikazu Setou, Mitsutoshi eng Research Support, Non-U.S. Gov't Technical Report 2009/10/01 Anal Chem. 2009 Nov 1; 81(21):9153-7. doi: 10.1021/ac901872n"