| Title: | The role of serotonergic signaling on phototactic and locomotor behavior in Daphnia magna |
| Author(s): | Gomez-Canela C; Esquius F; Barata C; |
| Address: | "Department of Analytical and Applied Chemistry, School of Engineering, Institut Quimic de Sarria-Universitat Ramon Llull, Barcelona, Spain. Department of Analytical and Applied Chemistry, School of Engineering, Institut Quimic de Sarria-Universitat Ramon Llull, Barcelona, Spain; Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA, CSIC), Jordi Girona 18, 08017 Barcelona, Spain. Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA, CSIC), Jordi Girona 18, 08017 Barcelona, Spain. Electronic address: cbmqam@cid.csic.es" |
| DOI: | 10.1016/j.scitotenv.2022.159042 |
| ISSN/ISBN: | 1879-1026 (Electronic) 0048-9697 (Linking) |
| Abstract: | "The role of serotonin in Daphnia magna phototactic and locomotor behavior was assessed using reverse genetics and pharmacological treatments with serotonin and fluoxetine. The study was conducted with four clones: the wild type clone and three CRISPR D. magna ones with mutations in the tryptophan hydrolase gene (TRH) that is involved in serotonin synthesis. These included clones TRHA- and TRHB- with mutations in both alleles that lack serotonin and the mono-allelic mutant TRH+, that has serotonin. Obtained results indicated that animals lacking serotonin showed an increased negative phototactism and locomotor activity upon light stimuli and a reduced response to fish kairomones relative to the wild type and TRH+ individuals. Exposure to exogenous serotonin re-established the phototactism and locomotor activity of TRH- individuals to those of the wild type but did not affect phototactic responses to fish kairomones. Unexpectedly, fluoxetine was able to modify locomotor activity and phototactic behavior against fish kairomones in TRH- individuals lacking serotonin, and also it increased the concentrations of acethylcholine and GABA in exposed animals, which support the argument that fluoxetine may also affect other neurological pathways" |
| Keywords: | Animals *Daphnia/physiology *Serotonin Fluoxetine Phototaxis Fishes Pheromones Tryptophan Behavior Daphnia Fish kairomones Serotonin; |
| Notes: | "MedlineGomez-Canela, Cristian Esquius, Ferran Barata, Carlos eng Netherlands 2022/09/30 Sci Total Environ. 2023 Jan 15; 856(Pt 2):159042. doi: 10.1016/j.scitotenv.2022.159042. Epub 2022 Sep 27" |
