| Title: | Terpenoid VOC profiles and functional characterization of terpene synthases in diploid and tetraploid cytotypes of Chrysanthemum indicum L |
| Author(s): | Feng L; Lin Y; Cai Y; Wei W; Yang J; Zhan R; Ma D; |
| Address: | "Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: rtzhan@gzucm.edu.cn. Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: madm@gzucm.edu.cn" |
| DOI: | 10.1016/j.plaphy.2023.107766 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Chrysanthemum indicum L. is a valuable medicinal plant with diploid and tetraploid forms that are widely distributed in central and southern China, and it contains abundant volatile organic compounds (VOCs). Despite the discovery of some terpene synthase (TPS) in C. indicum (i.e., CiTPS) in previous studies, many TPSs and their corresponding terpene biosynthesis pathways have yet to be discovered. In the present study, terpenoid VOCs in different tissues from two cytotypes of C. indicum were analyzed. We identified 52 types of terpenoid VOCs and systematically investigated the content and distribution of these compounds in various tissues. The two cytotypes of C. indicum exhibited different volatile terpenoid profiles. The content of monoterpenes and sesquiterpenes in the two cytotypes showed an opposite trend. In addition, four full-length candidate TPSs (named CiTPS5-8) were cloned from Ci-GD(4x), and their homologous TPS genes were screened based on the genome data of Ci-HB(2x). These eight TPSs displayed various tissue expression patterns and were discovered to produce 22 terpenoids, 5 of which are monoterpenes and 17 are sesquiterpenes. We further proposed corresponding terpene synthesis pathways, which can enable the establishment of an understanding of the volatile terpenoid profiles of C. indicum with different cytotypes. This knowledge may provide a further understanding of germplasm in C. indicum and may be useful for biotechnology applications of Chrysanthemum plants" |
| Keywords: | Terpenes/metabolism *Volatile Organic Compounds/metabolism Diploidy Tetraploidy *Chrysanthemum/genetics/metabolism Monoterpenes/metabolism *Alkyl and Aryl Transferases/genetics *Sesquiterpenes Chrysanthemum indicum L.Terpene synthase Terpenoid volatile o; |
| Notes: | "MedlineFeng, Lingfang Lin, Ying Cai, Yanjiao Wei, Wuke Yang, Jinfen Zhan, Ruoting Ma, Dongming eng France 2023/05/23 Plant Physiol Biochem. 2023 Jul; 200:107766. doi: 10.1016/j.plaphy.2023.107766. Epub 2023 May 16" |
