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Hortic Res

Title:		"Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes"
Author(s):		Du F; Wang T; Fan JM; Liu ZZ; Zong JX; Fan WX; Han YH; Grierson D;
Address:		"1College of Horticulture, Shanxi Agricultural University, 030801 Taigu, Shanxi China. ISNI: 0000 0004 1798 1300. GRID: grid.412545.3 2College of Horticulture, Nanjing Agricultural University, 210095 Nangjing, Jiangsu China. ISNI: 0000 0000 9750 7019. GRID: grid.27871.3b 3Experimental Teaching Center, Shanxi Agricultural University, 030801 Taigu, Shanxi China. ISNI: 0000 0004 1798 1300. GRID: grid.412545.3 4College of Agriculture, Shanxi Agricultural University, 030801 Taigu, Shanxi China. ISNI: 0000 0004 1798 1300. GRID: grid.412545.3 5Plant & Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD UK. ISNI: 0000 0004 1936 8868. GRID: grid.4563.4 6Department of Horticulture, College of Agriculture & Biotechnology, Zhejiang University, 310058 Hangzhou, China. ISNI: 0000 0004 1759 700X. GRID: grid.13402.34"
Journal Title:		Hortic Res
Year:		2019
Volume:		20191001
Issue:
Page Number:		110 -
DOI:		10.1038/s41438-019-0192-9
ISSN/ISBN:		2662-6810 (Print) 2052-7276 (Electronic) 2052-7276 (Linking)
Abstract:		"Lily is a well-known ornamental plant with a diversity of fragrant types. Basic information on lily floral scent compounds has been obtained for only a few accessions, and little is known about Lilium aroma types, the terpene synthase genes that may play roles in the production of key volatiles, or the range of monoterpenes that these genes produce. In this study, 41 cultivars were analyzed for volatile emissions, and a total of 46 individual volatile compounds were identified, 16 for the first time in lilies. Lily accessions were classified into six groups according to the composition of major scent components: faint-scented, cool, fruity, musky, fruity-honey, and lily. Monoterpenes were one of the main groups of volatiles identified, and attention was focused on terpene synthase (TPS) genes, which encode enzymes that catalyze the last steps in monoterpene synthesis. Thirty-two candidate monoterpene synthase cDNAs were obtained from 66 lily cultivars, and 64 SNPs were identified. Two InDels were also shown to result from variable splicing, and sequence analysis suggested that different transcripts arose from the same gene. All identified nucleotide substitution sites were highly correlated with the amounts of myrcene emitted, and InDel site 230 was highly correlated with the emission of all major monoterpenoid components, especially (E)-beta-ocimene. Heterologous expression of five cDNAs cloned from faint-scented and strong-scented lilies showed that their corresponding enzymes could convert geranyl diphosphate to (E)-beta-ocimene, alpha-pinene, and limonene. The findings from this study provide a major resource for the assessment of lily scent volatiles and will be helpful in breeding of improved volatile components"
Keywords:		Secondary metabolism Transcriptomics;
Notes:		"PubMed-not-MEDLINEDu, Fang Wang, Ting Fan, Jun-Miao Liu, Zhi-Zhi Zong, Jia-Xin Fan, Wei-Xin Han, Yuan-Huai Grierson, Donald eng England 2019/10/28 Hortic Res. 2019 Oct 1; 6:110. doi: 10.1038/s41438-019-0192-9. eCollection 2019"