« Previous AbstractVersatile Multi-Functional Block Copolymers Made by Atom Transfer Radical Polymerization and Post-Synthetic Modification: Switching from Volatile Organic Compound Sensors to Polymeric Surfactants for Water Rheology Control via Hydrolysis    Next AbstractA structurally deviant member of the Euplotes raikovi pheromone family: Er-23 »

Proc Natl Acad Sci U S A

Title:		Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level
Author(s):		Di Segni G; Gastaldi S; Zamboni M; Tocchini-Valentini GP;
Address:		"Cell Biology and Neurobiology Institute, Consiglio Nazionale delle Ricerche, Campus A. Buzzati-Traverso, 00015 Monterotondo, Rome, Italy"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2011
Volume:		20111003
Issue:		41
Page Number:		17082 - 17086
DOI:		10.1073/pnas.1114648108
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"The orderly expression of specific genes is the basis for cell differentiation. Saccharomyces cerevisiae has two haploid mating types, a and alpha cells, in which the mating-specific genes are differentially expressed. When a and alpha cells are committed to mate, their growth is arrested. Here we show that a cryptic polyadenylation site is present inside the coding region of the a-specific STE2 gene, encoding the receptor for the alpha-factor. The two cell types produce an incomplete STE2 transcript, but only a cells generate full-length STE2 mRNA. We eliminated the cryptic poly(A) signal, thereby allowing the production of a complete STE2 mRNA in alpha cells. We mutagenized alpha cells and isolated a mutant producing full-length STE2 mRNA. The mutation occurred in the ITC1 gene, whose product, together with the product of ISW2, is known to repress STE2 transcriptional initiation. We propose that the regulation of the yeast mating genes is achieved through a concerted mechanism involving transcriptional and posttranscriptional events. In particular, the early poly(A) site in STE2 could contribute to a complete shutoff of its expression in alpha cells, avoiding autocrine activation and growth arrest. Remarkably, no cryptic poly(A) sites are present in the a-factor receptor STE3 gene, indicating that S. cerevisiae has devised different strategies to regulate the two receptor genes. It is predictable that a correlation between the repression of a gene and the presence of a cryptic poly(A) site could also be found in other organisms, especially when expression of that gene may be harmful"
Keywords:		"Base Sequence Binding Sites/genetics Gene Expression Regulation, Fungal *Genes, Fungal Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Polyadenylation RNA, Fungal/genetics/metabolism RNA, Messenger/genetics/metabolism Receptors, Mating;"
Notes:		"MedlineDi Segni, Gianfranco Gastaldi, Serena Zamboni, Michela Tocchini-Valentini, Glauco P eng Research Support, Non-U.S. Gov't 2011/10/05 Proc Natl Acad Sci U S A. 2011 Oct 11; 108(41):17082-6. doi: 10.1073/pnas.1114648108. Epub 2011 Oct 3"