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J Cell Sci

Title:		Phosphorylation of Gbeta is crucial for efficient chemotropism in yeast
Author(s):		Deflorio R; Brett ME; Waszczak N; Apollinari E; Metodiev MV; Dubrovskyi O; Eddington D; Arkowitz RA; Stone DE;
Address:		"Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA"
Journal Title:		J Cell Sci
Year:		2013
Volume:		20130423
Issue:		Pt 14
Page Number:		2997 - 3009
DOI:		10.1242/jcs.112797
ISSN/ISBN:		1477-9137 (Electronic) 0021-9533 (Linking)
Abstract:		"Mating yeast cells interpret complex pheromone gradients and polarize their growth in the direction of the closest partner. Chemotropic growth depends on both the pheromone receptor and its associated G-protein. Upon activation by the receptor, Galpha dissociates from Gbetagamma and Gbeta is subsequently phosphorylated. Free Gbetagamma signals to the nucleus via a MAPK cascade and recruits Far1-Cdc24 to the incipient growth site. It is not clear how the cell establishes and stabilizes the axis of polarity, but this process is thought to require local signal amplification via the Gbetagamma-Far1-Cdc24 chemotropic complex, as well as communication between this complex and the activated receptor. Here we show that a mutant form of Gbeta that cannot be phosphorylated confers defects in directional sensing and chemotropic growth. Our data suggest that phosphorylation of Gbeta plays a role in localized signal amplification and in the dynamic communication between the receptor and the chemotropic complex, which underlie growth site selection and maintenance"
Keywords:		Aldehyde Oxidoreductases/metabolism Cell Cycle Proteins/metabolism Cell Polarity/genetics *Chemotaxis Cyclin-Dependent Kinase Inhibitor Proteins/metabolism GTP-Binding Protein alpha Subunits/*metabolism GTP-Binding Protein beta Subunits/genetics/*metaboli;
Notes:		"MedlineDeflorio, Reagan Brett, Marie-Elena Waszczak, Nicholas Apollinari, Elisabetta Metodiev, Metodi V Dubrovskyi, Oleksii Eddington, David Arkowitz, Robert A Stone, David E eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2013/04/25 J Cell Sci. 2013 Jul 15; 126(Pt 14):2997-3009. doi: 10.1242/jcs.112797. Epub 2013 Apr 23"