| Title: | The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor |
| Author(s): | Christensen PU; Davey J; Nielsen O; |
| Address: | "Department of Genetics, University of Copenhagen, Denmark" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe" |
| Keywords: | "ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics ATP-Binding Cassette Transporters/*genetics/*metabolism Amino Acid Sequence Base Sequence Cloning, Molecular DNA-Binding Proteins Fungal Proteins/genetics/metabolism Gene Expression Regulati;" |
| Notes: | "MedlineChristensen, P U Davey, J Nielsen, O eng Research Support, Non-U.S. Gov't Germany 1997/06/01 Mol Gen Genet. 1997 Jun; 255(2):226-36. doi: 10.1007/s004380050493" |
