| Title: | Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors |
| Author(s): | Castiglioni GZ; Bettio G; Matte CR; Jacques RA; Dos Santos Polidoro A; Rosa CA; Ayub MAZ; |
| Address: | "Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Goncalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil. Department of Microbiology, ICB, C.P. 486, State University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Goncalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br" |
| DOI: | 10.1007/s00449-020-02334-4 |
| ISSN/ISBN: | 1615-7605 (Electronic) 1615-7591 (Linking) |
| Abstract: | "Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 degrees C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L(-1) min(-1) in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L(-1) min(-1), using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min(-1) at 45 degrees C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC-MS as gamma-octalactone and gamma-nonalactone" |
| Keywords: | "Enzymes, Immobilized/*chemistry Geotrichum/*growth & development Hydrolysis Lipase/*chemistry Plant Oils/chemistry/*metabolism Seeds/*chemistry Vitis/*chemistry Volatile Organic Compounds/*metabolism Enzymatic hydrolysis Galactomyces geotrichum Geotrichum;" |
| Notes: | "MedlineCastiglioni, Gabriele Zanota Bettio, Giulia Matte, Carla Roberta Jacques, Rosangela Assis Dos Santos Polidoro, Allan Rosa, Carlos Augusto Ayub, Marco Antonio Zachia eng Financing Code 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ Germany 2020/03/25 Bioprocess Biosyst Eng. 2020 Aug; 43(8):1391-1402. doi: 10.1007/s00449-020-02334-4. Epub 2020 Mar 21" |
