| Title: | A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation |
| Author(s): | Capuano R; Spitalieri P; Talarico RV; Domakoski AC; Catini A; Paolesse R; Martinelli E; Novelli G; Sangiuolo F; Di Natale C; |
| Address: | "Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133, Rome, Italy. Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy. Department of Chemical Science and Technology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy. Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy. sangiuolo@med.uniroma2.it. Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133, Rome, Italy. dinatale@eln.uniroma2.it" |
| DOI: | 10.1038/s41598-017-01790-5 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application is the identification of residual pluripotent cells before cell transplantation to avoid the occurrence of teratomas. In this paper, we investigated in vitro the volatile compounds (VOCs) released during human induced pluripotent stem cells (hiPSCs) reprogramming. In particular, we studied hiPSCs differentiation to floating and adherent embryoid bodies until early neural progenitor cells. A preliminary Gas Chromatographic/Mass Spectrometer (GC/MS) analysis, based on a single extraction method and chromatographic separation, indicated 17 volatile compounds whose relative abundance is altered in each step of the differentiation process. The pattern of VOCs shown by hiPSCs is well distinct and makes these cells sharply separated from the other steps of differentiations. Similar behaviour has also been observed with an array of metalloporphyrins based gas sensors. The use of electronic sensors to control the process of differentiation of pluripotent stem cells might suggest a novel perspective for a fast and on-line control of differentiation processes" |
| Keywords: | "Cell Count *Cell Differentiation Cells, Cultured Electronic Nose Gas Chromatography-Mass Spectrometry Humans Induced Pluripotent Stem Cells/*cytology/*metabolism *Metabolome Principal Component Analysis Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlineCapuano, Rosamaria Spitalieri, Paola Talarico, Rosa Valentina Domakoski, Ana Carolina Catini, Alexandro Paolesse, Roberto Martinelli, Eugenio Novelli, Giuseppe Sangiuolo, Federica Di Natale, Corrado eng England 2017/05/11 Sci Rep. 2017 May 9; 7(1):1621. doi: 10.1038/s41598-017-01790-5" |
