| Title: | Wide transcriptional investigation unravel novel insights of the on-tree maturation and postharvest ripening of 'Abate Fetel' pear fruit |
| Author(s): | Busatto N; Farneti B; Tadiello A; Oberkofler V; Cellini A; Biasioli F; Delledonne M; Cestaro A; Noutsos C; Costa F; |
| Address: | "1Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy. ISNI: 0000 0004 1755 6224. GRID: grid.424414.3 2Department of Biology, University of Padova, Via G. Colombo 3, 35121 Padova, Italy. ISNI: 0000 0004 1757 3470. GRID: grid.5608.b Institute for Biochemistry and Biology, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany. 4Department of Agricultural and Food Science, University of Bologna, Via Fanin 46, 40127 Bologna, Italy. ISNI: 0000 0004 1757 1758. GRID: grid.6292.f 5Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy. ISNI: 0000 0004 1755 6224. GRID: grid.424414.3 6Department of Biotecnology, University of Verona, Strada le Grazie 15, Ca Vignal 1, 37134 Verona, Italy. ISNI: 0000 0004 1763 1124. GRID: grid.5611.3 7Unit of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy. ISNI: 0000 0004 1755 6224. GRID: grid.424414.3 8Biology Department, SUNY College at Old Westbury, Old Westbury, NY 11568 USA. ISNI: 0000 0000 9827 7702. GRID: grid.264271.4" |
| DOI: | 10.1038/s41438-018-0115-1 |
| ISSN/ISBN: | 2662-6810 (Print) 2052-7276 (Electronic) 2052-7276 (Linking) |
| Abstract: | "To decipher the transcriptomic regulation of the on-tree fruit maturation in pear cv. 'Abate Fetel', a RNA-seq transcription analysis identified 8939 genes differentially expressed across four harvesting stages. These genes were grouped into 11 SOTA clusters based on their transcriptional pattern, of which three included genes upregulated while the other four were represented by downregulated genes. Fruit ripening was furthermore investigated after 1 month of postharvest cold storage. The most important variation in fruit firmness, production of ethylene and volatile organic compounds were observed after 5 days of shelf-life at room temperature following cold storage. The role of ethylene in controlling the ripening of 'Abate Fetel' pears was furthermore investigated through the application of 1-methylcyclopropene, which efficiently delayed the progression of ripening by reducing fruit softening and repressing both ethylene and volatile production. The physiological response of the interference at the ethylene receptor level was moreover unraveled investigating the expression pattern of 12 candidate genes, initially selected to validate the RNA-seq profile. This analysis confirmed the effective role of the ethylene competitor in downregulating the expression of cell wall (PG) and ethylene-related genes (ACS, ACO, ERS1, and ERS2), as well as inducing one element involved in the auxin signaling pathway (Aux/IAA), highlighting a possible cross-talk between these two hormones. The expression patterns of these six elements suggest their use as molecular toolkit to monitor at molecular level the progression of the fruit on-tree maturation and postharvest ripening" |
| Notes: | "PubMed-not-MEDLINEBusatto, Nicola Farneti, Brian Tadiello, Alice Oberkofler, Vicky Cellini, Antonio Biasioli, Franco Delledonne, Massimo Cestaro, Alessandro Noutsos, Christos Costa, Fabrizio eng England 2019/03/12 Hortic Res. 2019 Mar 1; 6:32. doi: 10.1038/s41438-018-0115-1. eCollection 2019" |
