| Title: | Identification of a hyperactive mating pheromone of Saccharomyces cerevisiae |
| Author(s): | Caldwell GA; Wang SH; Dawe AL; Naider F; Becker JM; |
| Address: | "Department of Microbiology, University of Tennessee, Knoxville 37996" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "The yeast mating pheromone a-factor is a farnesylated peptide [YIIKGVFWDPAC(Farnesyl)-OCH3] involved in the signal transduction cascade which leads to sexual conjugation of haploid cells. We have identified a synthetic analog of the a-factor, [D-Ala5] a-factor, which exhibits 4-6 fold greater biological activity than that of a-factor as judged by two different assay systems. In contrast, [L-Ala5] a-factor has 4-16 fold lower activity than wild-type a-factor. [D-Ala5] a-factor remains susceptible to cleavage by the yeast alpha-cell specific protease a-factorase, thereby ruling out increased activity due to greater stability. This report describes the first example of a hyperactive S. cerevisiae pheromone and may suggest a conformationally preferred form of this lipopeptide ligand" |
| Keywords: | "Amino Acid Sequence Conjugation, Genetic/drug effects Haploidy Kinetics Mating Factor Molecular Sequence Data Oligopeptides/chemical synthesis/metabolism/*pharmacology Peptides/chemical synthesis/metabolism/*pharmacology Pheromones/*pharmacology Saccharom;" |
| Notes: | "MedlineCaldwell, G A Wang, S H Dawe, A L Naider, F Becker, J M eng GM46520/GM/NIGMS NIH HHS/ Comparative Study Research Support, U.S. Gov't, P.H.S. 1993/12/30 Biochem Biophys Res Commun. 1993 Dec 30; 197(3):1173-8. doi: 10.1006/bbrc.1993.2600" |
