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J Am Soc Mass Spectrom

Title:		Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry
Author(s):		Boshier PR; Marczin N; Hanna GB;
Address:		"Department of Biosurgery and Surgical Technology, Imperial College London, St. Mary's Hospital, London, United Kingdom"
Journal Title:		J Am Soc Mass Spectrom
Year:		2010
Volume:		20100210
Issue:		6
Page Number:		1070 - 1074
DOI:		10.1016/j.jasms.2010.02.008
ISSN/ISBN:		1879-1123 (Electronic) 1044-0305 (Linking)
Abstract:		"Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the repeatability of the analysis of volatile metabolites within the breath of healthy volunteers, with emphasis on the influence of sampling methodology. Baseline instrument specific coefficients of variability for examined metabolites were as follows: acetone (1%), ammonia (1%), isoprene (2%), propanol (6%), ethanol (7%), acetic acid (7%), and hydrogen cyanide (19%). Metabolite concentration and related product ion count rate were identified as strong determinants of measurement variation. With the exception of ammonia, an orally released metabolite, variability in repeated on-line breath analysis tended to be lower for metabolites of systemic origin. Standardization of sampling technique improved the repeatability of the analysis of selected metabolites. Off-line (bag) alveolar breath sampling, as opposed to mixed (whole) breath sampling, likewise improved the repeatability of the analysis of all metabolites investigated, with the exception of acetic acid. We conclude that SIFT-MS analysis of common volatile metabolites within the breath of healthy volunteers is both reliable and repeatable. For selected metabolites, the finding that repeatability is improved through modification of sampling methodology may have implications in terms of future recommended practices"
Keywords:		1-Propanol/analysis Acetic Acid/analysis Acetone/analysis Adult Ammonia/analysis Analysis of Variance Breath Tests/*methods Butadienes/analysis Ethanol/analysis Exhalation Female Hemiterpenes/analysis Humans Hydrogen Cyanide/analysis Male Mass Spectrometr;
Notes:		"MedlineBoshier, Piers R Marczin, Nandor Hanna, George B eng Research Support, Non-U.S. Gov't 2010/03/26 J Am Soc Mass Spectrom. 2010 Jun; 21(6):1070-4. doi: 10.1016/j.jasms.2010.02.008. Epub 2010 Feb 10"