| Title: | Microbial cell-free extracts as sources of enzyme activities to be used for enhancement flavor development of ewe milk cheese |
| Author(s): | Calasso M; Mancini L; Di Cagno R; Cardinali G; Gobbetti M; |
| Address: | "Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy. Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy. Electronic address: raffaella.dicagno@uniba.it. Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy" |
| ISSN/ISBN: | 1525-3198 (Electronic) 0022-0302 (Linking) |
| Abstract: | "Freeze-dried cell-free extracts (CFE) from Lactobacillus casei LC01, Weissella cibaria 1XF5, Hafnia alvei Moller ATCC 51815, and Debaryomyces hansenii LCF-558 were used as sources of enzyme activities for conditioning the ripening of ewe milk cheese. Compared with control cheese (CC), CFE did not affect the gross composition and the growth of the main microbial groups of the cheeses. As shown through urea-PAGE electrophoresis of the pH 4.6-soluble nitrogen fraction and the analysis of free AA, the secondary proteolysis of the cheeses with CFE added was markedly differed from that of the CC. Compared with CC, several enzyme activities were higher in the water-soluble extracts from cheeses made with CFE. In agreement, the levels of 49 volatile compounds significantly differentiated CC from the cheeses made with CFE. The level of some alcohols, ketones, sulfur compounds, and furans were the lowest in the CC, whereas most aldehydes were the highest. Each CFE seemed to affect a specific class of chemical compounds (e.g., the CFE from H. alvei ATCC 51815 mainly influenced the synthesis of sulfur compounds). Apart from the microbial source used, the cheeses with the addition of CFE showed higher score for acceptability than the control cheese. Cheese ripening was accelerated or conditioned using CFE as sources of tailored enzyme activities" |
| Keywords: | Adult Alcohols/analysis Aldehydes/analysis Animals Cheese/analysis/*microbiology Female Food Handling/methods Food Microbiology Furans/analysis Hafnia alvei/metabolism Humans Hydrogen-Ion Concentration Ketones/analysis Lacticaseibacillus casei/metabolism; |
| Notes: | "MedlineCalasso, Maria Mancini, Leonardo Di Cagno, Raffaella Cardinali, Gianluigi Gobbetti, Marco eng Randomized Controlled Trial 2015/07/06 J Dairy Sci. 2015 Sep; 98(9):5874-89. doi: 10.3168/jds.2015-9362. Epub 2015 Jul 2" |
