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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Rapid discrimination of fungal strains isolated from human skin based on microbial volatile organic profiles
Author(s):		Belinato JR; Silva E; de Souza DS; Marco PH; Valderrama P; do Prado RM; Bonugli-Santos RC; Pilau EJ; Porto C;
Address:		"Department of Chemistry, State University of Maringa (UEM), Ave. Colombo 5790, Maringa, PR 87020-900, Brazil. Postgraduate Program in Food Technology (PPGTA), Federal University of Technology - Parana (UTFPR), P.O. Box, 271, Campo Mourao, PR 87301-899, Brazil. Animal Science Department, State University of Maringa (UEM), Ave. Colombo 5790, Maringa, PR 87020-900, Brazil. Federal University for Latin American Integration (UNILA), Institute Latin American of Nature and Life Sciences (ILACNV), Av Taquinio Joslin dos Santos, 1000, Foz do Iguacu 85870-650, PR, Brazil. Department of Chemistry, State University of Maringa (UEM), Ave. Colombo 5790, Maringa, PR 87020-900, Brazil. Electronic address: epilau@uem.br. Department of Chemistry, State University of Maringa (UEM), Ave. Colombo 5790, Maringa, PR 87020-900, Brazil; Program of Master in Science, Technology and Food Safety, Cesumar Institute of Science, Technology and Innovation (ICETI), University Center of Maringa - (UNICESUMAR), Av. Guedner, 1610, Maringa, PR 87050-900, Brazil"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2019
Volume:		20190210
Issue:
Page Number:		9 - 14
DOI:		10.1016/j.jchromb.2019.02.007
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"Studies of the microbiota of human skin have gained attention mainly because of its high complexity. Volatile metabolites that emerge from the microbiota play a significant role in fungus metabolism, acting on fungal development, defense, and protection against stress, communication, and pathogenicity. The present study evaluated volatile organic profiles, based on headspace-solid-phase microextraction-gas chromatography-mass spectrometry. We sought to define the optimal experimental conditions for such identification. Chromatograms from 15 fungi were evaluated and discriminated by principal component analysis. The volatile metabolite profiles that were putatively identified in the present study (e.g 2?ª╡isopropyl?ª╡5?ª╡methyl?ª╡cyclohex?ª╡3?ª╡en?ª╡1?ª╡one, 3/2?ª╡methyl?ª╡1?ª╡butanol, isopentyl ethanoate, phenyl ethanol) allowed the discrimination of different microorganisms from human skin. The present methodology may be a more rapid way of identifying microorganisms compared with conventional methods of microbiological identification"
Keywords:		Fungi/*chemistry/classification/isolation & purification Gas Chromatography-Mass Spectrometry/*methods Humans Mycological Typing Techniques/*methods Principal Component Analysis Skin/*microbiology Solid Phase Microextraction Volatile Organic Compounds/*an;
Notes:		"MedlineBelinato, Joao Raul Silva, Evandro de Souza, Daiane Silva Marco, Paulo Henrique Valderrama, Patricia do Prado, Rodolpho Martin Bonugli-Santos, Rafaella C Pilau, Eduardo Jorge Porto, Carla eng Netherlands 2019/02/19 J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Mar 15; 1110-1111:9-14. doi: 10.1016/j.jchromb.2019.02.007. Epub 2019 Feb 10"