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Plant Cell Environ

Title:		Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis
Author(s):		Ameztoy K; Baslam M; Sanchez-Lopez AM; Munoz FJ; Bahaji A; Almagro G; Garcia-Gomez P; Baroja-Fernandez E; De Diego N; Humplik JF; Ugena L; Spichal L; Dolezal K; Kaneko K; Mitsui T; Cejudo FJ; Pozueta-Romero J;
Address:		"Instituto de Agrobiotecnologia, Consejo Superior de Investigaciones Cientificas/Gobierno de Navarra, Avenida Pamplona 123, Mutilva, Navarra, 31192, Spain. Laboratory of Biochemistry, Faculty of Agriculture, Niigata University, Niigata, 950-2181, Japan. Department of Chemical Biology and Genetics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Olomouc, CZ-78371, Czech Republic. Instituto de Bioquimica Vegetal y Fotosintesis, Universidad de Sevilla and Consejo Superior de Investigaciones Cientificas, Seville, 41092, Spain"
Journal Title:		Plant Cell Environ
Year:		2019
Volume:		20190710
Issue:		9
Page Number:		2627 - 2644
DOI:		10.1111/pce.13601
ISSN/ISBN:		1365-3040 (Electronic) 0140-7791 (Linking)
Abstract:		"Microorganisms produce volatile compounds (VCs) that promote plant growth and photosynthesis through complex mechanisms involving cytokinin (CK) and abscisic acid (ABA). We hypothesized that plants' responses to microbial VCs involve posttranslational modifications of the thiol redox proteome through action of plastidial NADPH-dependent thioredoxin reductase C (NTRC), which regulates chloroplast redox status via its functional relationship with 2-Cys peroxiredoxins. To test this hypothesis, we analysed developmental, metabolic, hormonal, genetic, and redox proteomic responses of wild-type (WT) plants and a NTRC knockout mutant (ntrc) to VCs emitted by the phytopathogen Alternaria alternata. Fungal VC-promoted growth, changes in root architecture, shifts in expression of VC-responsive CK- and ABA-regulated genes, and increases in photosynthetic capacity were substantially weaker in ntrc plants than in WT plants. As in WT plants, fungal VCs strongly promoted growth, chlorophyll accumulation, and photosynthesis in ntrc-Delta2cp plants with reduced 2-Cys peroxiredoxin expression. OxiTRAQ-based quantitative and site-specific redox proteomic analyses revealed that VCs promote global reduction of the thiol redox proteome (especially of photosynthesis-related proteins) of WT leaves but its oxidation in ntrc leaves. Our findings show that NTRC is an important mediator of plant responses to microbial VCs through mechanisms involving global thiol redox proteome changes that affect photosynthesis"
Keywords:		"Abscisic Acid/metabolism *Alternaria Arabidopsis/*drug effects/metabolism Arabidopsis Proteins/*metabolism Cytokinins/metabolism Photosynthesis/drug effects Protein Processing, Post-Translational/*drug effects Proteome Thioredoxin-Disulfide Reductase/*met;"
Notes:		"MedlineAmeztoy, Kinia Baslam, Marouane Sanchez-Lopez, Angela Maria Munoz, Francisco Jose Bahaji, Abdellatif Almagro, Goizeder Garcia-Gomez, Pablo Baroja-Fernandez, Edurne De Diego, Nuria Humplik, Jan F Ugena, Lydia Spichal, Lukas Dolezal, Karel Kaneko, Kentaro Mitsui, Toshiaki Cejudo, Francisco Javier Pozueta-Romero, Javier eng Research Support, Non-U.S. Gov't 2019/06/22 Plant Cell Environ. 2019 Sep; 42(9):2627-2644. doi: 10.1111/pce.13601. Epub 2019 Jul 10"