| Title: | Volatile organic compounds and good laboratory practices in the in vitro fertilization laboratory: the important parameters for successful outcome in extended culture |
| Author(s): | Agarwal N; Chattopadhyay R; Ghosh S; Bhoumik A; Goswami SK; Chakravarty B; |
| Address: | "Department of Assisted Reproduction, Institute of Reproductive Medicine, HB-36/A/3, Salt Lake City, Sector-III, Kolkata, West Bengal, 700106, India. Department of Assisted Reproduction, Institute of Reproductive Medicine, HB-36/A/3, Salt Lake City, Sector-III, Kolkata, West Bengal, 700106, India. bncirm@gmail.com" |
| DOI: | 10.1007/s10815-017-0947-x |
| ISSN/ISBN: | 1573-7330 (Electronic) 1058-0468 (Print) 1058-0468 (Linking) |
| Abstract: | "PURPOSE: This study aims to describe the role of implementing good laboratory practices to improve in vitro fertilization (IVF) outcomes which are of great interest for practitioners dealing with infertility. METHODS: Certain modifications were introduced in May 2015 in our IVF laboratory like high-efficiency particulate air CODA system, steel furniture instead of wooden, use of new disinfectants like oosafe, and restriction of personnel entry along with avoidance of cosmetics like perfume to improve pregnancy rates. Volatile organic compound (VOC) meter reading was monitored at two time points and five different places in the laboratory to compare the embryonic development parameters before (group A: July 2014-April 2015) and after (group B: July 2015-April 2016) remodeling. RESULTS: The IVF outcomes from 1036 cycles were associated in this study. Reduction in VOC meter readings, enhanced air quality, improvement in blastocyst formation rate, implantation, and clinical pregnancy rate were observed in the laboratory after implementation of new facilities. Results illustrated that the attention must be focused on potential hazards which expose laboratories to elevated VOC levels. Blastocyst formation rate increased around 18%. Implantation rate, clinical pregnancy rate, and live birth rate increased by around 11, 10, and 8%, respectively. CONCLUSION: In conclusion, with proper engineering and material selection, we have been able to reduce chemical contamination and adverse effects on culture with optimized IVF results. SUPPORT: None" |
| Keywords: | Adult Birth Rate Blastocyst/*cytology/metabolism Embryo Implantation/*physiology Embryo Transfer/methods Embryonic Development/physiology Female Fertilization in Vitro/methods Humans Infertility/metabolism/physiopathology Laboratories Pregnancy Pregnancy; |
| Notes: | "MedlineAgarwal, Nupur Chattopadhyay, Ratna Ghosh, Sanghamitra Bhoumik, Arpita Goswami, S K Chakravarty, Baidyanath eng Observational Study Netherlands 2017/05/26 J Assist Reprod Genet. 2017 Aug; 34(8):999-1006. doi: 10.1007/s10815-017-0947-x. Epub 2017 May 24" |
