| Title: | Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori |
| Author(s): | Zhang TY; Kang L; Zhang ZF; Xu WH; |
| Address: | "Department of Molecular and Cell Biology, University of Science and Technology of China, Hefei 230027, People's Republic of China" |
| ISSN/ISBN: | 1470-8728 (Electronic) 0264-6021 (Print) 0264-6021 (Linking) |
| Abstract: | "Diapause hormone (DH) and PBAN (pheromone biosynthesis-activating neuropeptide) are two important insect neuropeptides regulating development and reproduction respectively. In the present study, we report two Bombyx mori transcription factors interacting specifically with the promoter of Bom-DH-PBAN (where Bom-DH stands for B. mori DH); we named them DHMBP-1 and -2 (DH-modulator-binding proteins 1 and 2). The developmental changes of DHMBP-1/-2 are closely correlated with that of Bom-DH-PBAN mRNA throughout the pupal stage. Competition assays indicate that DHMBP-1 from Chinese B. mori possesses binding characteristics similar to those of the POU-M1 protein from Japanese B. mori. POU-M1 cDNAs were cloned from various tissues of Chinese B. mori and were found to be distinct from the previously published POU-M1 in amino acid residues 108-136 because of insertion mutations. Owing to this difference in amino acid residues, we named this cDNA POU-M2. Even though POU-M2 differs from POU-M1 at the N-terminal, the POU domain and the binding properties of both POU-M1 and -M2 are the same. Functional analysis showed that overexpression of POU-M2 in the Bombyx cell line BmN activated the promoter of Bom-DH-PBAN, but failed to activate a promoter in which the POU-binding element was mutated. The transcriptional activity of POU-M2 is probably regulated by other factors binding to the upstream of the promoter sequence. We show that the POU-M2-binding site was able to activate the transcription of a heterologous promoter of the gene encoding B. mori larval serum protein. POU-M1 was found to exhibit the same transcriptional activities as POU-M2. Taken together, these results demonstrate that POU-M2 plays an important role in the transcriptional regulation of the Bom-DH-PBAN gene" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Bombyx/growth & development/*metabolism DNA-Binding Proteins/chemistry Gene Expression Regulation Genes, Reporter Insect Proteins/*biosynthesis/chemistry/genetics/*isolation & purification/physiology Larva Molecul;" |
| Notes: | "MedlineZhang, Tian-Yi Kang, Le Zhang, Zhi-Fang Xu, Wei-Hua eng Comparative Study Research Support, Non-U.S. Gov't England 2004/02/10 Biochem J. 2004 May 15; 380(Pt 1):255-63. doi: 10.1042/BJ20031482" |
